HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
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Issued Date
2022-01-01
Resource Type
ISSN
19848250
eISSN
21759790
Scopus ID
2-s2.0-85142427836
Journal Title
Brazilian Journal of Pharmaceutical Sciences
Volume
58
Rights Holder(s)
SCOPUS
Bibliographic Citation
Brazilian Journal of Pharmaceutical Sciences Vol.58 (2022)
Suggested Citation
Sunthudlakhar P., Sithisarn P., Rojsanga P., Jarikasem S. HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand. Brazilian Journal of Pharmaceutical Sciences Vol.58 (2022). doi:10.1590/s2175-97902022e20656 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86891
Title
HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Phellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1-10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099-0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms.