Gene Profiling of Cannabis-sativa-mediated Apoptosis in Human Melanoma Cells
2
Issued Date
2023-03-01
Resource Type
ISSN
02507005
eISSN
17917530
Scopus ID
2-s2.0-85149153741
Pubmed ID
36854502
Journal Title
Anticancer Research
Volume
43
Issue
3
Start Page
1221
End Page
1237
Rights Holder(s)
SCOPUS
Bibliographic Citation
Anticancer Research Vol.43 No.3 (2023) , 1221-1237
Suggested Citation
Poommarapan K., Rummaneethorn P., Srisubat A., Suwanpidokkul N., Leenutaphong P., Nararatwanchai T., Srihirun S., Phetchengkao W., Suriyachan K., Tancharoen S. Gene Profiling of Cannabis-sativa-mediated Apoptosis in Human Melanoma Cells. Anticancer Research Vol.43 No.3 (2023) , 1221-1237. 1237. doi:10.21873/anticanres.16269 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81641
Title
Gene Profiling of Cannabis-sativa-mediated Apoptosis in Human Melanoma Cells
Other Contributor(s)
Abstract
Background/Aim: Malignant melanoma is an aggressive skin cancer, accounting for the majority of skin cancer deaths. Prognosis is often poor and finding effective treatment remains a challenge. Tetrahydrocannabinol (THC) and cannabidiol (CBD) are main bioactive components of Cannabis sativa plant extracts that have been shown to exert anti-tumor effects. In this study, we aimed to perform gene expression analysis of human melanoma A375 cells following stimulation with C. sativa extracts. Materials and Methods: Gene expression profiles of A375 human melanoma and Vero (control) cell lines were evaluated by RNA sequencing and quantitative real-time PCR. Results: Flow cytometry showed that the THC+CBD cannabis fractions induced apoptosis on A375 cells. Induction of apoptosis was accompanied by a notable up-regulation of DNA damage inducible transcript 3 (DDIT), nerve growth factor receptor (NGFR), colony-stimulating factor 2 (CSF2), growth arrest and DNA damage inducible beta (GADD45B), and thymic stromal lymphopoietin (TSLP) genes and down-regulation of aryl hydrocarbon receptor nuclear translocator 2 (ARNT2), cyclin E2 (CCNE2), integrin subunit alpha 9 (ITGA9), proliferating cell nuclear antigen (PCNA) and E2F transcription factor 1 (E2F1) genes. Treatment of A375 cells with the THC+CBD fraction inhibited the phosphorylation of ERK1/2 signaling pathway, which regulates melanoma cell proliferation. We showed that the THC+CBD combination disrupted melanoma cell migration. Conclusion: Use of C. sativa-derived extracts containing equal amounts of THC and CBD is proposed as a potential treatment of melanoma.