Isolation and identification of a newly isolated lipase-producing bacteria (Acinetobacter baumannii RMUTT3S8-2) from oily wastewater treatment pond in a poultry processing factory and its optimum lipase production
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Issued Date
2022-12-01
Resource Type
eISSN
2589014X
Scopus ID
2-s2.0-85141478236
Journal Title
Bioresource Technology Reports
Volume
20
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bioresource Technology Reports Vol.20 (2022)
Suggested Citation
Bunmadee S., Teeka J., Lomthong T., Kaewpa D., Areesirisuk P., Areesirisuk A. Isolation and identification of a newly isolated lipase-producing bacteria (Acinetobacter baumannii RMUTT3S8-2) from oily wastewater treatment pond in a poultry processing factory and its optimum lipase production. Bioresource Technology Reports Vol.20 (2022). doi:10.1016/j.biteb.2022.101267 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84030
Title
Isolation and identification of a newly isolated lipase-producing bacteria (Acinetobacter baumannii RMUTT3S8-2) from oily wastewater treatment pond in a poultry processing factory and its optimum lipase production
Other Contributor(s)
Abstract
This research aimed to optimize the cultural conditions of a newly isolated RMUTT3S8-2 from oily wastewater treatment pond in a poultry processing factory to enhance its lipase production. The isolated RMUTT3S8-2 was molecularly identified as Acinetobacter baumannii. Lipase production was optimized using Plackett-Burman Design and Box-Behnken Design. The important variables were investigated and chosen as peptone, 24.49 g/L; yeast extract, 33.82 g/L and NaCl, 6.21 g/L. In optimized conditions, the lipase activity of A. baumannii RMUTT3S8-2 was 216.23 ± 3.69 U/mL, more than unoptimized condition by 2.2 times. Lipase stability was above 95 % in the pH range of 5.0 to 9.0 at 30 °C. In addition, A. baumannii RMUTT3S8-2 could digest the oil in poultry processing wastewater and exhibited the degree of oil hydrolysis of 41.94 ± 4.98 % at 12 days. This newly isolated A. baumannii RMUTT3S8-2 showed promise as a lipase producer, especially for oily wastewater treatment.