Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves
Issued Date
2024-12-01
Resource Type
ISSN
2085191X
eISSN
23387610
Scopus ID
2-s2.0-85218728811
Journal Title
Biosaintifika
Volume
16
Issue
3
Start Page
427
End Page
436
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biosaintifika Vol.16 No.3 (2024) , 427-436
Suggested Citation
Hardjo P.H., Askitosari T.D., Wijaya A.N., Edbert J., Artadana I.B.M., Wittayathanarattana T. Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves. Biosaintifika Vol.16 No.3 (2024) , 427-436. 436. doi:10.15294/biosaintifika.v16i3.12057 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/105573
Title
Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves
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Corresponding Author(s)
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Abstract
Acehnese Patchouli var. Sidikalang can produce patchouli oil, one of the country's sources of foreign exchange, and it is advantageous in many industries. However, patchouli alcohol (PA) content in patchouli oil is still low. The higher PA content determines the better quality of patchouli oil. So, delignification with Trichoderma viride was carried out to increase PA and yield of patchouli oil. The duration of delignification patchouli leaves is one factor affecting the increase in PA and yield. This study aimed to determine the effect of delignification duration on PA content and yield. The research method was completely randomized with 8 treatments (a combination of 2 treatments: with and without delignification, and 4 delignification durations: 0, 3, 6, and 9 days). The inoculum used for delignification was an inoculum ball, and then patchouli leaf samples were extracted using microwave-assisted extraction (MAE). Gas chromatography was carried out for PA analysis. Delignification of patchouli leaves, with a delignification time of 9 days, showed high PA and yield, which were (0.3129 ± 0.1557)% and (1.4543 ± 0.7717)%, respectively. The success of delignification at 9 days was also shown by increased phenolic content. Trichoderma viride is often utilized in delignification process, but the optimal duration of its delignification of Aceh patchouli leaves that can help improve patchouli oil quality is not yet known. This research is hoped to help related industries improve the secondary metabolites of plants, which has many benefits for the community. Implication/benefit for science development/society.