Recombinant Production of Food Allergens in Yeast Pichia pastoris
Issued Date
2024-01-01
Resource Type
eISSN
19406029
Scopus ID
2-s2.0-85171955272
Pubmed ID
37737976
Journal Title
Methods in molecular biology (Clifton, N.J.)
Volume
2717
Start Page
29
End Page
40
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in molecular biology (Clifton, N.J.) Vol.2717 (2024) , 29-40
Suggested Citation
Piboonpocanun S. Recombinant Production of Food Allergens in Yeast Pichia pastoris. Methods in molecular biology (Clifton, N.J.) Vol.2717 (2024) , 29-40. 40. doi:10.1007/978-1-0716-3453-0_3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90284
Title
Recombinant Production of Food Allergens in Yeast Pichia pastoris
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
The methylotrophic yeast Pichia pastoris (P. pastoris) is one of the expression systems widely used to produce recombinant heterologous proteins. In this chapter, the methodology to produce recombinant food allergens in P. pastoris is described. The methodology begins with the preparation of competent P. pastoris cells followed by the transformation of the competent cells by electroporation as well as the preparation of plasmid DNA for transformation. Moreover, the screening of yeast transformants by direct PCR to ensure integration of allergen DNA followed by small-scale expression of recombinant allergen in yeast cells is also described.