Recombinant Production of Food Allergens in Yeast Pichia pastoris
8
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2024-01-01
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item.page.dc.identifier.eissn
19406029
item.page.dc.identifier.doi
item.page.dc.identifier.scopus
2-s2.0-85171955272
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37737976
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Methods in molecular biology (Clifton, N.J.)
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2717
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29
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40
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SCOPUS
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Methods in molecular biology (Clifton, N.J.) Vol.2717 (2024) , 29-40
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Piboonpocanun S. Recombinant Production of Food Allergens in Yeast Pichia pastoris. Methods in molecular biology (Clifton, N.J.) Vol.2717 (2024) , 29-40. 40. doi:10.1007/978-1-0716-3453-0_3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90284
item.page.dc.title
Recombinant Production of Food Allergens in Yeast Pichia pastoris
item.page.dc.contributor.author
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item.page.dc.description.abstract
The methylotrophic yeast Pichia pastoris (P. pastoris) is one of the expression systems widely used to produce recombinant heterologous proteins. In this chapter, the methodology to produce recombinant food allergens in P. pastoris is described. The methodology begins with the preparation of competent P. pastoris cells followed by the transformation of the competent cells by electroporation as well as the preparation of plasmid DNA for transformation. Moreover, the screening of yeast transformants by direct PCR to ensure integration of allergen DNA followed by small-scale expression of recombinant allergen in yeast cells is also described.