Optimization of Natural Deep Eutectic Solvent-Assisted Extraction of Rosmarinic Acid from Thunbergia laurifolia Lindl. and Evaluation of Antioxidant Activity
Issued Date
2025-12-01
Resource Type
eISSN
14203049
Scopus ID
2-s2.0-105025758978
Journal Title
Molecules
Volume
30
Issue
24
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecules Vol.30 No.24 (2025)
Suggested Citation
Kriengsaksri K., Thongphichai W., Uttarawichien T., Khoochonthara J., Towiwat P., Sukrong S. Optimization of Natural Deep Eutectic Solvent-Assisted Extraction of Rosmarinic Acid from Thunbergia laurifolia Lindl. and Evaluation of Antioxidant Activity. Molecules Vol.30 No.24 (2025). doi:10.3390/molecules30244795 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113736
Title
Optimization of Natural Deep Eutectic Solvent-Assisted Extraction of Rosmarinic Acid from Thunbergia laurifolia Lindl. and Evaluation of Antioxidant Activity
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Abstract
Thunbergia laurifolia Lindl. is a plant known for its promising biological activity, including antioxidant and anti-inflammatory activities, and a rich source of rosmarinic acid (RA). The extraction of T. laurifolia for cosmetic and skincare products using conventional solvents has encountered difficulties, including safety concerns, skin irritation, undesirable odors, and inefficient extraction. In this work, 14 types of natural deep eutectic solvents (NaDESs) with varying compositions and ratios were investigated to compare their efficiency in extracting RA from T. laurifolia by HPLC analysis. The NaDES with the highest extraction efficiency was further utilized in ultrasonic-assisted extraction (UAE), and the extraction parameters were optimized using response surface methodology. The optimized RA content and DPPH scavenging activity were predicted by response surfaces methodology to be 7.52 mg/g DW and 37.6 mg TE/g DW, respectively. The optimal extraction condition was achieved using a propylene glycol-lactic acid NaDES (at an 8:1 molar ratio) with 37% (w/w) H<inf>2</inf>O, a 30 mL/g liquid-to-solid ratio, an 80 °C extraction temperature, and a 32 min extraction time. The optimized extract was proved to suppress ROS in H<inf>2</inf>O<inf>2</inf>-induced keratinocytes. The extract demonstrated robust stability against basic, oxidative, and photolytic stresses, and maintained long-term chemical stability up to 90 days. This study introduces a new green solvent for the effective extraction of T. laurifolia, thereby improving the safety and quality of the extracts for skincare and cosmetic products.