Application of QuantiFERON ELISA for Detection of Interferon-Gamma Autoantibodies in Adult-Onset Immunodeficiency Syndrome
Issued Date
2022-01-01
Resource Type
ISSN
00075027
eISSN
19437730
Scopus ID
2-s2.0-85123389813
Pubmed ID
34463313
Journal Title
Lab Medicine
Volume
53
Issue
1
Start Page
12
End Page
17
Rights Holder(s)
SCOPUS
Bibliographic Citation
Lab Medicine Vol.53 No.1 (2022) , 12-17
Suggested Citation
Khositnithikul R. Application of QuantiFERON ELISA for Detection of Interferon-Gamma Autoantibodies in Adult-Onset Immunodeficiency Syndrome. Lab Medicine Vol.53 No.1 (2022) , 12-17. 17. doi:10.1093/labmed/lmab039 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86700
Title
Application of QuantiFERON ELISA for Detection of Interferon-Gamma Autoantibodies in Adult-Onset Immunodeficiency Syndrome
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Objective: Patients who develop interferon-gamma autoantibodies (IFN-γ autoAbs) in adult-onset immunodeficiency (AOID) syndrome are more likely to develop opportunistic and recurrent intracellular infections. The assay to detect IFN-γ autoAbs is essential for the diagnosis and therapeutic monitoring of AOID syndrome. Therefore, this study applied the QuantiFERON assay for the detection of IFN-γ autoAbs. Methods: Serum from patients with AOID syndrome (n = 19) and serum from healthy patients (n = 20) was collected and applied using 2 neutralizing platforms of enzyme-linked immunosorbent assay (ELISA) kits (the BD ELISA and the QuantiFERON ELISA) for IFN-γ autoAbs detection. Results: The pooled serum from patients with AOID syndrome showed >50% inhibition at 1:5000 dilution (positive), whereas the pooled serum from healthy patients showed <50% inhibition at 1:5000 dilution (negative) according to the neutralizing QuantiFERON ELISA. Each specimen showed the same result according to both the neutralizing BD ELISA and the neutralizing QuantiFERON ELISA. Moreover, the patient serum showed a variation in titer ranging from 1:5000 to >1:5,000,000 according to the neutralizing QuantiFERON ELISA. Conclusion: The QuantiFERON ELISA kit could be applied for the detection of IFN-γ autoAbs for the diagnosis and therapeutic monitoring of AOID syndrome.