Scanning elecatron microscopic study of the splenic vascular east of common tree shrew (Tupaia glis)
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
viii, 57 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Anatomy))--Mahidol University, 1989
Suggested Citation
Sirinush Bamroongwong Scanning elecatron microscopic study of the splenic vascular east of common tree shrew (Tupaia glis). Thesis (M.Sc. (Anatomy))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100151
Title
Scanning elecatron microscopic study of the splenic vascular east of common tree shrew (Tupaia glis)
Alternative Title(s)
การศึกษาโครงหลอดเลือดภายในม้ามของกระแตด้วยจุลทรรศน์อิเลกตรอนแบบสแกน
Author(s)
Advisor(s)
Abstract
The splenic vascular casts of the common tree shrew were consturcted with Batsons no. 17 compound and view under SEM for the study of detail features of microvasculature, with complementary usage of vinyl casting method for the study of gross arterial system in relation to venous system. Adult common tree shrew of both sexes weighing between 120 g and 180 g were divided into 2 groups to be injected with red and blue vinyl resin, and plastic mixture (Batsons no. 17), respectively. Under ether anaesthesia, 0.05 ml of heparin was injected, the right atrium was cut open, and 250 ml of 0.9% NaC1 solution was perfused, both through the left ventricle, following with 50 ml of 10% neutral formalin solution. Then resin or plastic mixture was perfused mannually through the left ventricle, until it was flowing out from the right atrium. After hardening, the vinyl resin and plastic mixture injected specimens were macarated in 40% KOH. After waching in water and drying at room temperature, the vinyl resin corrosion casts were studied under stereomicroscope. The plastic corrosion casts of the spleen were dissected free, coated with carbon and gold before viewing under SEM at 15 kV. It was found that the spleen of common tree shrew was supplied by splenic artery which divided into trabecular arteries. The trabecular arteries divided again into central arterioles. Kach central arteriole sent out 15 to 30 rediating arterioles; celled penicillar arteriole. After numerous divisions and became smaller, they entered marginal zone and red pulp. Here each arteriole continued directly into sinusoids. The size of the sinusoid was 10.5 um. (7.0 to 14.0 um) which was larger than ordinary capillaries in the same animal; that is 4.2 um (3.3 to 5.0 um). The sinusoids emptied into pulp venules and passed into trabecular veins. The trabecular veins traveled to the hilum, where they drained into splenic veins and finally drained into portal vein. It is most likely that the splenic circulation of the common tree shrew is the closed circulation type.
Description
Anatomy (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Anatomy
Degree Grantor(s)
Mahidol University