Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling
Issued Date
2024-03-01
Resource Type
eISSN
20797737
Scopus ID
2-s2.0-85188681008
Journal Title
Biology
Volume
13
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biology Vol.13 No.3 (2024)
Suggested Citation
Soommat P., Raethong N., Ruengsang R., Thananusak R., Laomettachit T., Laoteng K., Saithong T., Vongsangnak W. Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling. Biology Vol.13 No.3 (2024). doi:10.3390/biology13030139 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97811
Title
Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling
Corresponding Author(s)
Other Contributor(s)
Abstract
The genome-scale metabolic model (GSMM) of Cordyceps militaris provides a comprehensive basis of carbon assimilation for cell growth and metabolite production. However, the model with a simple mass balance concept shows limited capability to probe the metabolic responses of C. militaris under light exposure. This study, therefore, employed the transcriptome-integrated GSMM approach to extend the investigation of C. militaris’s metabolism under light conditions. Through the gene inactivity moderated by metabolism and expression (GIMME) framework, the iPS1474-tiGSMM model was furnished with the transcriptome data, thus providing a simulation that described reasonably well the metabolic responses underlying the phenotypic observation of C. militaris under the particular light conditions. The iPS1474-tiGSMM obviously showed an improved prediction of metabolic fluxes in correlation with the expressed genes involved in the cordycepin and carotenoid biosynthetic pathways under the sucrose culturing conditions. Further analysis of reporter metabolites suggested that the central carbon, purine, and fatty acid metabolisms towards carotenoid biosynthesis were the predominant metabolic processes responsible in light conditions. This finding highlights the key responsive processes enabling the acclimatization of C. militaris metabolism in varying light conditions. This study provides a valuable perspective on manipulating metabolic genes and fluxes towards the target metabolite production of C. militaris.