Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation
Issued Date
2023-09-01
Resource Type
ISSN
17732247
Scopus ID
2-s2.0-85166348480
Journal Title
Journal of Drug Delivery Science and Technology
Volume
87
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Drug Delivery Science and Technology Vol.87 (2023)
Suggested Citation
Saimok W., Iyaraganjanakul P., Kreeporn P., Phuanghom W., Sa-ngiamsuntorn K., Hongeng S., Nasongkla N. Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation. Journal of Drug Delivery Science and Technology Vol.87 (2023). doi:10.1016/j.jddst.2023.104784 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88272
Title
Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation
Author's Affiliation
Other Contributor(s)
Abstract
Hepatocyte encapsulation serves as an alternative solution for prolonging the hepatic pre-transplantation period in acute liver disease patients. The basic fibroblast growth factor (bFGF) was introduced and optimized to enhance hepatocyte viability and regeneration at 1 ng/ml concentrations. The bFGF was preserved with double-emulsion PLGA microspheres to release over a period of time sustainably. Different techniques were used to calculate the percentages of loading content, encapsulation efficiency, and release profile. In addition, the bFGF-loaded microspheres were measured to be 1.8226 ± 0.7176 μm in size and illustrated smooth surface morphology. Herein, the immortalized hepatocyte-like cell line (imHC) and bFGF-loaded PLGA microspheres were encapsulated inside the alginate microbeads using the electrostatic extrusion method. Under the scanning electron microscope, the bead morphology was observed to have a smooth surface, and the estimated size was 758 ± 32.9 μm. Moreover, the confocal images showed the evenly distributed cells inside the microbeads in a 3D structure. Over a 14-day observation, the microsphere exhibited significant effects on imHC viability. Therefore, it can be concluded that the co-encapsulation of bFGF-loaded microspheres and imHCs inside alginate microbeads (bFGF-HB) has presented itself as a promising approach to enhance the cell survivability of encapsulated cells.