Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation
16
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2023-09-01
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17732247
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2-s2.0-85166348480
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Journal of Drug Delivery Science and Technology
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87
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SCOPUS
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Journal of Drug Delivery Science and Technology Vol.87 (2023)
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Saimok W., Iyaraganjanakul P., Kreeporn P., Phuanghom W., Sa-ngiamsuntorn K., Hongeng S., Nasongkla N. Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation. Journal of Drug Delivery Science and Technology Vol.87 (2023). doi:10.1016/j.jddst.2023.104784 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88272
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Co-encapsulation of bFGF-loaded microspheres and hepatocytes in microbeads for prolonging hepatic pre-transplantation
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Hepatocyte encapsulation serves as an alternative solution for prolonging the hepatic pre-transplantation period in acute liver disease patients. The basic fibroblast growth factor (bFGF) was introduced and optimized to enhance hepatocyte viability and regeneration at 1 ng/ml concentrations. The bFGF was preserved with double-emulsion PLGA microspheres to release over a period of time sustainably. Different techniques were used to calculate the percentages of loading content, encapsulation efficiency, and release profile. In addition, the bFGF-loaded microspheres were measured to be 1.8226 ± 0.7176 μm in size and illustrated smooth surface morphology. Herein, the immortalized hepatocyte-like cell line (imHC) and bFGF-loaded PLGA microspheres were encapsulated inside the alginate microbeads using the electrostatic extrusion method. Under the scanning electron microscope, the bead morphology was observed to have a smooth surface, and the estimated size was 758 ± 32.9 μm. Moreover, the confocal images showed the evenly distributed cells inside the microbeads in a 3D structure. Over a 14-day observation, the microsphere exhibited significant effects on imHC viability. Therefore, it can be concluded that the co-encapsulation of bFGF-loaded microspheres and imHCs inside alginate microbeads (bFGF-HB) has presented itself as a promising approach to enhance the cell survivability of encapsulated cells.