Plant cell and tissue cultures for the production of medicine, cosmetic and food ingredients
Issued Date
2018
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
Faculty of Pharmacy Mahidol University
Graduate School of Pharmaceutical Sciences Chiba University
Graduate School of Pharmaceutical Sciences Chiba University
Suggested Citation
Somnuk Bunsupa, Veena Nukoolkarn, Pongtip Sithisarn, Nitirat Visetkit, Pornpatsorn Lertphadungkit, Paktraporn Mekloy, Piyanuch Rojsanga, Krisada Sakchaisri, Supaart Sirikantaramas (2018). Plant cell and tissue cultures for the production of medicine, cosmetic and food ingredients. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25184
Title
Plant cell and tissue cultures for the production of medicine, cosmetic and food ingredients
Other Contributor(s)
Abstract
Plant tissue culture is the technique of maintaining and growing plant cells, tissues or organs
on artificial medium under defined physical and chemical conditions in vitro. Different plant
tissue culture systems have been extensively studied to improve and enhance the production
of plant chemicals in various medicinal plants. The aim of our study is to utilize plant tissue
culture techniques for the production of medicine, cosmetic and food ingredients by using
Thai medicinal plants. We selected three plants including Trichosanthes cucumerina L.,
Moringa oleifera Lam. and Oroxylum indicum (L.) Benth. ex Kurz based on information
from Thai traditional medicine. The callus from those plants was induced by using suitable
phytohormones and growth conditions. Subsequently, the cell suspension culture was
established by agitation of callus the Erlenmeyer flask containing suitable liquid media.
Various elicitors such as methyl jasmonate, chiotosan and yeast extract were added to cell
suspension culture aiming to increase the production of desired compounds. The contents of
compounds were analyzed by using high performance liquid chromatography (HPLC)
together with the determination of total phenolic and total flavonoid contents. The biological
activities, such as antioxidant and cytotoxicity were also tested. In addition, we also aim to
study the biosynthetic pathway of bryonolic acid in Tricosanthes cucumerina by combining
the metabolite profiles and expression of the genes from transcriptomic data to narrow down
the candidate genes involved in the biosynthetic pathway. Furthermore, we aim to clone,
characterize and produce MRK protein which is a potent anti HIV-I reverse transcriptase
from Thai bitter melon (Momordica charantia). In this topic, I will present the recent
progress of our study and perspective about utilizing plant tissue culture as an alternative
source for the production of valuable secondary metabolites.
Description
The 3rd Chiba University-Mahidol University Joint Symposium
on Pharmaceutical Sciences. Hosted by Faculty of Pharmacy, Mahidol University
Thursday August 2, 2018