Vaginal microbiome of women with adenomyosis: A case-control study
Issued Date
2022-02-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85124779565
Pubmed ID
35171931
Journal Title
PLoS ONE
Volume
17
Issue
2 February
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.17 No.2 February (2022)
Suggested Citation
Kunaseth J., Waiyaput W., Chanchaem P., Sawaswong V., Permpech R., Payungporn S., Sophonsritsuk A. Vaginal microbiome of women with adenomyosis: A case-control study. PLoS ONE Vol.17 No.2 February (2022). doi:10.1371/journal.pone.0263283 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86620
Title
Vaginal microbiome of women with adenomyosis: A case-control study
Other Contributor(s)
Abstract
Immune dysregulation can involve invasion and survival of endometrial glands inside the myometrium of the adenomyosis. There is limited available data concerning alterations of the bacterial microbiome in the reproductive tract of adenomyosis women. The present cross-sectional age-matched study aims to compare vaginal microbiota between women with and without adenomyosis. We recruited women with adenomyosis (N = 40) and age-matched women without adenomyosis (N = 40) from the Departments of Obstetrics and Gynaecology, Ramathibodi Hospital Mahidol University, from August 2020 to January 2021. Vaginal swab samples were collected from the participants. DNA isolation and bacterial 16s rDNA gene sequencing and data analyses were then performed. Comparison of the diversity of vaginal microbiota, microbiota composition, and the operational taxonomic unit (OTU) between adenomyosis and non-adenomyosis (control) groups were undertaken. Data from 40 and 38 women with and without adenomyosis, respectively, were analyzed. Alpha-diversity analysis (Chao1 index) at the species level showed higher vaginal microbial richness in the adenomyosis group when compared with the control group (p = 0.006). The linear discriminant analysis effect size technique (LeFSe) indicated an elevated abundance of several vaginal microbial taxa in the adenomyosis group, including Alloscardovia, Oscillospirales, Ruminoccoccaceae, UCG_002, Oscillospiraceae, Enhydrobacter, Megamonas, Moraxellaceae, Subdoligranulum, Selenomonadaceae, and Faecalibacterium. On the other hand, an increase in the abundance of Megaspehera, Fastidiosipila, Hungateiclostridiaceae, and Clostridia was identified in the control group. Vaginal community state type (CST)-III and -IV were dominated in adenomyosis, while only CST-IV was dominated in the non-adenomyosis group. Lactobacillus was the most abundant vaginal microbial in both groups. In this study, the differences in vaginal microbiome profile were noted between adenomyosis and non-adenomyosis group. The increasing of microbial richness was associated with adenomyosis. Nevertheless, further investigations were required to elucidate the mechanisms and apply them for clinical implications.