Effects of stevioside and steviol on absorptive function of hamster intestine
2
Issued Date
2024
Copyright Date
1993
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiv, 176 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Physiology))--Mahidol University, 1993
Suggested Citation
Monthaporn Suteerawattananon Effects of stevioside and steviol on absorptive function of hamster intestine. Thesis (M.Sc. (Physiology))--Mahidol University, 1993. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99841
Title
Effects of stevioside and steviol on absorptive function of hamster intestine
Alternative Title(s)
การศึกษาถึงผลของสตีวิโอไซด์และสตีวิออลต่อการดูดซึมสารอาหารในลำไส้ของหนูแฮมสเตอร์
Author(s)
Abstract
The effects of stevioside and steviol (a product of enzymatic hydrolysis of stevioside) on intestinal glucose absorption was examined in hamsters jejunum. By using jejunal slices technique, it was indicated that stevioside (1 and 5 mM) had no inhibitory effect on glucose absorption in hamsters jejunum in vitro. In contrast, steviol (0.1-2.0 mM) inhibited glucose absorption with highly significant value. The possible mechanism by which steviol had an inhibitory effect on intestinal glucose absorption in vitro was investigated by using everted sac technique. Steviol (2 mM) at 60 minutes of incubation caused the inhibition up to 44.27%. The inhibition of glucose absorption was not due to the reduction in intestinal mucosal Na(+)-K(+)-ATPase activity. It was shown that the inhibitory action of steviol was associated with the reduction of mucosal ATP content in intestinal absorptive cells. The inhibition of NADH cytochrome c reductase activity by steviol (2 mM) was detected with the degree of 30.43% and cytochrome oxidase with the degree of 28.22%. The inhibitory effect of steviol on brush-border membrane was not found. The effect of steviol on morphological changes of jejunal mucosa was also investigated. The shortening of jejunal villi and undulating epithelial lining were remarkably appeared. The lacteal of villi core are dilated. The crypts and villi epithelial cells are degenerated and then appeared like large round vacuole. The inhibitory effect of stevioside on intestinal glucose absorption was found in hamsters given with stevioside at the dose of 2.5 g/kg BW/day for 12 weeks. No inhibition was observed in hamsters given with stevioside at the doses of 0.5 and 1.0 g/kg BW/day with the same period. The mucosal Na(+)-K(+)-ATPase activity was reduced in hamsters given with stevioside 2.5 g/kg BW/day for 12 weeks by 23.72%. The reduction of intestinal mucosal ATP content was also found in this dose of stevioside and the percent inhibition was 20.65% Additionally, treatment of stevioside at the doses of 1.0 and 2.5 g/kg BW/day caused an increase in sucrase activity of hamsters jejunum with statistically significant. It was noted-that stevioside at the doses of 1.0 and 2.5 g/kg BW/day for 12 weeks caused the slightly shortening villi and undulating epithelial lining. In conclusion, the present study show that only steviol had an inhibitory effect on intestinal glucose absorption in vitro. Such an inhibition is partly due to the reduction of intestinal mucosal ATP content. And the reduction in mucosal ATP content is a consequence of the reduction of intestinal mitochondrial respiratory enzymes activities and the morphological changes of intestinal absorptive cells. In vivo studies, the inhibition of glucose absorption by subchronic feeding of stevioside is caused by the inhibition of intestinal mucosal Na(+)-K(+)-ATPa se activity, the reduction of mucosal ATP content and the changes in morphology of intestinal absorptive cells.
Description
Physiology (Mahidol University 1993)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Physiology
Degree Grantor(s)
Mahidol University