Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications
Issued Date
2022-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85141184643
Pubmed ID
36329229
Journal Title
Scientific Reports
Volume
12
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.12 No.1 (2022)
Suggested Citation
Benchaprathanphorn K., Sakulaue P., Siriwatwechakul W., Muangman P., Chinaroonchai K., Namviriyachote N., Viravaidya-Pasuwat K. Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications. Scientific Reports Vol.12 No.1 (2022). doi:10.1038/s41598-022-21913-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86386
Title
Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications
Other Contributor(s)
Abstract
Cell sheet engineering, a scaffold-free approach to fabricate functional tissue constructs from several cell monolayers, has shown promise in tissue regeneration and wound healing. Unfortunately, these cell sheets are often too small to provide sufficient wound area coverage. In this study, we describe a process to enlarge cell sheets using MEEK micrografting, a technique extensively used to expand skin autografts for large burn treatments. Human dermal fibroblast cell sheets were placed on MEEK’s prefolded gauze without any use of adhesive, cut along the premarked lines and stretched out at various expansion ratios (1:3, 1:6 and 1:9), resulting in regular distribution of many square islands of fibroblasts at a much larger surface area. The cellular processes essential for wound healing, including reattachment, proliferation, and migration, of the fibroblasts on expanded MEEK gauze were superior to those on nylon dressing which served as a control. The optimal expansion ratio with the highest migration rate was 1:6, possibly due to the activation of chemical signals caused by mechanical stretching and an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications.