Inhibition of LPS-Induced Microglial Activation by the Ethyl Acetate Extract of Pueraria mirifica
1
Issued Date
2022-10-01
Resource Type
ISSN
16617827
eISSN
16604601
Scopus ID
2-s2.0-85139748435
Pubmed ID
36232220
Journal Title
International Journal of Environmental Research and Public Health
Volume
19
Issue
19
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Environmental Research and Public Health Vol.19 No.19 (2022)
Suggested Citation
Jantaratnotai N., Thampithak A., Utaisincharoen P., Pinthong D., Sanvarinda P. Inhibition of LPS-Induced Microglial Activation by the Ethyl Acetate Extract of Pueraria mirifica. International Journal of Environmental Research and Public Health Vol.19 No.19 (2022). doi:10.3390/ijerph191912920 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84675
Title
Inhibition of LPS-Induced Microglial Activation by the Ethyl Acetate Extract of Pueraria mirifica
Author's Affiliation
Other Contributor(s)
Abstract
Microglial activation has been found to play a crucial role in various neurological disorders. Proinflammatory substances overproduced by activated microglia, such as cytokines, chemokines, reactive oxygen species, and nitric oxide (NO), can result in neuroinflammation that further exacerbates the course of the diseases. This study aimed to explore the anti-inflammatory effect of the ethyl acetate extract of Pueraria mirifica on microglial activation. Lipopolysaccharide (LPS)-induced inflammation was used as a model to investigate the effects of P. mirifica on HAPI (highly aggressive proliferating immortalized), a rat microglial cell line. Administration of ethyl acetate extract from the tuberous roots of P. mirifica to HAPI cells dose-dependently reduced NO production and iNOS expression induced by LPS. Attenuation of IRF-1 (interferon regulatory factor-1) induction, one of the transcription factors governing iNOS expression, suggested that the inhibitory effect on NO production by the plant extract was at least partially mediated through this transcription factor. In addition, LPS-stimulated mRNA expression of MCP-1 (monocyte chemoattractant protein-1), IL-6 (interleukin-6), and TNF-α (tumor necrosis factor-α) was also suppressed with P. mirifica extract pretreatment. This study indicates that the ethyl acetate extract of P. mirifica could potentially serve as an anti-inflammatory mediator and may be useful in relieving the severity of neurological diseases where microglia play a role.