Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains
1
Issued Date
2024-01-01
Resource Type
ISSN
16878639
eISSN
16878647
Scopus ID
2-s2.0-85186070990
Journal Title
Advances in Virology
Volume
2024
Rights Holder(s)
SCOPUS
Bibliographic Citation
Advances in Virology Vol.2024 (2024)
Suggested Citation
Kongchanagul A., Masrinoul P., Boonarkart C., Suptawiwat O., Auewarakul P. Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains. Advances in Virology Vol.2024 (2024). doi:10.1155/2024/5691673 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97464
Title
Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains
Corresponding Author(s)
Other Contributor(s)
Abstract
Hemagglutinin (HA) is the major envelope glycoprotein and antigen on the surface of influenza virions. The glycoprotein comprises a globular head and a stalk region. While immunodominant epitopes on influenza HA head are highly variable, the stalk domain is conserved. The variability of the HA head causes the antigenic drift that made the requirement of annual update of vaccine strains. Induction of antibody against the stalk domain has been proposed as an approach for a broadly protective influenza vaccine strategy. Sequential exposure to influenza strains with highly diverse HA heads but conserved stalks have been shown to induce antibody to the low immunogenic stalk domain. Here, we tested this approach by using old influenza vaccine strains that are decades apart in evolution. Inactivated whole virion vaccine of influenza A/Puerto Rico/8/1934, A/USSR/92/1977, and A/Thailand/102/2009 (H1N1) was sequentially immunized into BALB/c mice in comparison to immunization using single strain (A/Thailand/102/2009 (H1N1)). The sequentially immunized mice developed higher levels of binding antibody to the stalk domain. These suggested that using old vaccine strains in sequential vaccination may be a possible approach to induce antibody to the conserved stalk domain.