Difference of seminal plasma and sperm proteins in good and poor freezability boar ejaculates
Issued Date
2022-01-01
Resource Type
ISSN
03507149
eISSN
18491170
Scopus ID
2-s2.0-85115621157
Journal Title
Veterinarska Stanica
Volume
53
Issue
2
Start Page
425
End Page
438
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinarska Stanica Vol.53 No.2 (2022) , 425-438
Suggested Citation
Rungruangsak J., Suwimonteerabutr J., Buranaamnuay K., Asawakarn S., Chantavisoote N., Pisitkun T., Chaweewan K., Tummaruk P. Difference of seminal plasma and sperm proteins in good and poor freezability boar ejaculates. Veterinarska Stanica Vol.53 No.2 (2022) , 425-438. 438. doi:10.46419/VS.53.2.8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87104
Title
Difference of seminal plasma and sperm proteins in good and poor freezability boar ejaculates
Other Contributor(s)
Abstract
The present study was performed to compare the expression of sperm proteins, i.e. triosephosphate isomerase (TPI) and acrosin binding protein (ACRBP) and seminal plasma proteins, i.e. glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), in boar semen with good, moderate and poor freezability. The study was conducted by determining the protein contents in 32 sperm samples and 38 seminal plasma samples of semen. The ejaculated semen was divided into two portions: the first portion was centrifuged to separate the pellet of sperm from the seminal plasma and the second portion was cryopreserved. After thawing, the ejaculates were classified into three groups according to their post-thawed sperm motility: good (60.2 ± 1.7%), moderate (29.3 ± 2.0%) and poor (16.6 ± 2.2%) freezabilities. The expressions of GPX5 and FN1 in seminal plasma and TPI and ACRBP in sperm were determined using Western blot analysis. It was found that, for sperm proteins, the level of TPI was negatively correlated with the post-thawed total sperm motility (r = -0.38, P = 0.029). For seminal plasma proteins, the level of FN1 in the seminal plasma was positively correlated with the post-thawed total sperm motility (r = 0.37, P = 0.021) and progressive motility (r = 0.39, P = 0.016). The expression of GPX5 was not correlated with any of the frozen–thawed sperm qualities (P > 0.05). In conclusions, boar semen containing a high level of FN1 in seminal plasma has better freezability. Frozen–thawed sperm motility was positively correlated with the level of FN1 in boar seminal plasma and negatively correlated with TPI in boar spermatozoa.