Colorimetric Sensing of Gram-Negative and Gram-Positive Bacteria Using 4-Mercaptophenylboronic Acid-Functionalized Gold Nanoparticles in the Presence of Polyethylene Glycol
Issued Date
2023-04-11
Resource Type
eISSN
24701343
Scopus ID
2-s2.0-85151366436
Journal Title
ACS Omega
Volume
8
Issue
14
Start Page
13456
End Page
13464
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Omega Vol.8 No.14 (2023) , 13456-13464
Suggested Citation
Amornwairat P., Pissuwan D. Colorimetric Sensing of Gram-Negative and Gram-Positive Bacteria Using 4-Mercaptophenylboronic Acid-Functionalized Gold Nanoparticles in the Presence of Polyethylene Glycol. ACS Omega Vol.8 No.14 (2023) , 13456-13464. 13464. doi:10.1021/acsomega.3c01205 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81537
Title
Colorimetric Sensing of Gram-Negative and Gram-Positive Bacteria Using 4-Mercaptophenylboronic Acid-Functionalized Gold Nanoparticles in the Presence of Polyethylene Glycol
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Gold nanoparticles (GNPs) have been used as detection probes for rapid and sensitive detection of various analytes, including bacteria. Here, we demonstrate a simple strategy for bacterial detection using GNPs functionalized with 4-mercaptophenylboronic acid (4-MPBA). 4-MPBA can interact with peptidoglycan or lipopolysaccharides present in bacterial organelles. After the addition of a high concentration of sodium hydroxide (NaOH), the functionalization of the surface of 50 nm GNPs with 4-MPBA (4-MPBA@GNPs) in the presence of polyethylene glycol results in a color change because of the aggregation of 4-MPBA@GNPs. This color change is dependent on the amount of bacteria present in the tested samples. Escherichia coli (E. coli) K-12 and Staphylococcus aureus (S. aureus) are used as Gram-negative and Gram-positive bacterial models, respectively. The color change can be detected within an hour by the naked eye. A linear relationship is observed between bacterial concentrations and the absorbance intensity at 533 nm; R2 values of 0.9152 and 0.8185 are obtained for E. coli K-12 and S. aureus, respectively. The limit of detection of E. coli K-12 is ∼2.38 × 102 CFU mL-1 and that of S. aureus is ∼4.77 × 103 CFU mL-1. This study provides a promising approach for the rapid detection of target Gram-negative and Gram-positive bacteria.