Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer
4
Issued Date
2025-05-01
Resource Type
ISSN
23529520
Scopus ID
2-s2.0-105001493176
Journal Title
OpenNano
Volume
23
Rights Holder(s)
SCOPUS
Bibliographic Citation
OpenNano Vol.23 (2025)
Suggested Citation
Dana P., Taweechaipaisankul A., Wongngam Y., Sungsuwan S., Chonniyom W., Klibaim S., Tanyapanyachon P., Rattanatayarom M., Phoraksa O., Asawapirom U., Punnakitikashem P., Polpanich D., Saengkrit N. Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer. OpenNano Vol.23 (2025). doi:10.1016/j.onano.2025.100242 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/109343
Title
Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer
Corresponding Author(s)
Other Contributor(s)
Abstract
Kaempferia galanga L. (KGL) is an aromatic ginger that has been used as a medicinal plant. Specifically, KGL possesses anti-inflammatory, antioxidant, anti-bacterial, and anti-cancer effects. However, the key component of KGL, ethyl p‑methoxy cinnamate (EPMC), is insoluble in water, resulting in low bioavailability. Hence, a nano-drug delivery system is used to enhance KGL activities. This study aimed to employ polydopamine (PDA) nanoparticles as a carrier for KGL delivery to improve its anti-cancer activity against colorectal cancer cells. PDA- and PDA nanoparticle–loaded KGL (PDA-KGL) were synthesized using a spontaneous oxidation process. The physicochemical properties of the PDA-KGL were characterized by dynamic light scattering methods. The anti-cancer activity of PDA-KGL was evaluated in HT-29, a colorectal cancer (CRC) cell line. Average hydrodynamic sizes of PDA and PDA-KGL were 236.2 ± 1.2 and 316.6 ± 2.0 nm, respectively, and the zeta potential of PDA and PDA-KGL were -23.0 ± 0.4 and -39.5 ± 0.6 mV, respectively. The morphology of PDA-KGL observed under TEM was spherical in shape. Anti-proliferative activity was monitored in HT-29 cells using MTT and 3D tumor spheroid assays. PDA-KGL strongly inhibited cell viability of HT-29 cells compared to free KGL and PDA treatments. PDA-KGL induced apoptosis in HT-29 cells as shown by an Annexin V binding assay. In addition, PDA-KGL suppressed the invasive ability of HT-29 cells compared to free KGL or PDA, which was determined by a transwell invasion assay. Taken together, it implies that PDA-KGL might be used as a nano-drug delivery approach for colorectal cancer treatment.