Enhancing intestinal tight junction assembly by gallic acid as a subcellular basis for the pharmacological effect of Ocimum sanctum L. flower aqueous extract
Issued Date
2024-11-01
Resource Type
ISSN
17564646
Scopus ID
2-s2.0-85207910120
Journal Title
Journal of Functional Foods
Volume
122
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Functional Foods Vol.122 (2024)
Suggested Citation
Wachiradejkul W., Sukmak P., Treveeravoot S., Yurasakpong L., Rangchaikul N., Chatkul P., Supapol P., Arinno A., Teansuk N., Inchai J., Phummisutthigoon S., Phongjit M., Loungjan A., Akrimajirachoote N., Poolsri W., Aonbangkhen C., Khumjiang R., Muanprasat C., Vaddhanaphuti C.S., Pongkorpsakol P. Enhancing intestinal tight junction assembly by gallic acid as a subcellular basis for the pharmacological effect of Ocimum sanctum L. flower aqueous extract. Journal of Functional Foods Vol.122 (2024). doi:10.1016/j.jff.2024.106519 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101948
Title
Enhancing intestinal tight junction assembly by gallic acid as a subcellular basis for the pharmacological effect of Ocimum sanctum L. flower aqueous extract
Author's Affiliation
Bangkokthonburi University
Faculty of Science, Mahidol University
Center of Excellence on Petrochemical and Materials Technology
Faculty of Medicine, Chiang Mai University
Chulalongkorn University
Chulabhorn Royal Academy
Kasetsart University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Burapha University
Laboratory of Epithelial Tight Junction Pathophysiology
In Silico and Clinical Anatomy Research Group (iSCAN)
Faculty of Science, Mahidol University
Center of Excellence on Petrochemical and Materials Technology
Faculty of Medicine, Chiang Mai University
Chulalongkorn University
Chulabhorn Royal Academy
Kasetsart University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Burapha University
Laboratory of Epithelial Tight Junction Pathophysiology
In Silico and Clinical Anatomy Research Group (iSCAN)
Corresponding Author(s)
Other Contributor(s)
Abstract
Intestinal tight junction disruption initiates pathogenesis of colitis and determines diseases progression. At present, there is no therapeutics that directly corrects intestinal tight junction disassembly. Here, we discovered that Ocimum sanctum L. flower aqueous extract (OSLF) and gallic acid, increased intestinal barrier function by suppressing tight junction-dependent leak pathway permeability after being disrupted by Ca2+ depletion method. Transepithelial electrical resistance (TER) measurement, western blot analysis, immunofluorescence staining, and molecular docking indicated that OSLF and gallic acid improved intestinal barrier function by inducing tight junction assembly and inhibiting leak pathway permeability in intestinal epithelial cell monolayers via CaMKK-β/AMPK/SIRT-1/ERK-dependent fashion. Therefore, Gallic acid represents a drug candidate for treating diseases associated impaired intestinal barrier function including colitis.