Transcriptome analysis reveals a role of FOXO3 in antileukemia/lymphoma properties of panduratin A
Issued Date
2024-10-22
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85206968970
Pubmed ID
39433897
Journal Title
Scientific reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific reports Vol.14 No.1 (2024) , 24795
Suggested Citation
Phuagkhaopong S., Janpattanapichai J., Sirirak N., Khemawoot P., Vivithanaporn P., Suknuntha K. Transcriptome analysis reveals a role of FOXO3 in antileukemia/lymphoma properties of panduratin A. Scientific reports Vol.14 No.1 (2024) , 24795. doi:10.1038/s41598-024-75630-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101776
Title
Transcriptome analysis reveals a role of FOXO3 in antileukemia/lymphoma properties of panduratin A
Corresponding Author(s)
Other Contributor(s)
Abstract
Boesenbergia rotunda, commonly known as fingerroot, is a medicinal and culinary plant native to the Indochina Peninsula. We found that panduratin A (Pan-A), one of the compounds present in the rhizome extract of fingerroot, inhibited cell proliferation, induced apoptosis, and promoted cell cycle arrest at G0/G1 phase in multiple hematologic malignant cell lines including leukemia and lymphoma lines. Pan-A inhibited these activities in leukemia and lymphoma cells in a concentration-dependent manner. High-throughput transcriptome analysis indicated that Pan-A is involved in the cell cycle, cellular senescence, apoptosis, and multiple canonical signaling pathways in lymphoma cells. The Forkhead box O (FOXO) transcription factor family was identified as a potential target of Pan-A. Western blot showed elevated caspase 7 and cPARP/PARP in the B-cell lymphoma cells after treatment with Pan-A. The inhibitory effects were accompanied by stimulation of Akt signaling and phosphorylation of FOXO3. Immunohistochemistry of tissues from patients with B-cell lymphoma revealed detectable levels of FOXO3 protein specifically in neoplastic B cells. Overall, our results highlight FOXO3 as a player underlying antileukemia/lymphoma effects of Pan-A.