Expression of VEGF165 and VEGF165b during ovarian follicular development
Issued Date
2023-11-01
Resource Type
ISSN
23050500
eISSN
23050519
Scopus ID
2-s2.0-85181511339
Journal Title
Asian Pacific Journal of Reproduction
Volume
12
Issue
6
Start Page
281
End Page
287
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Reproduction Vol.12 No.6 (2023) , 281-287
Suggested Citation
Changsangfa C., Turathum B., Sroyraya M., Grataitong K. Expression of VEGF165 and VEGF165b during ovarian follicular development. Asian Pacific Journal of Reproduction Vol.12 No.6 (2023) , 281-287. 287. doi:10.4103/2305-0500.390303 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/95945
Title
Expression of VEGF165 and VEGF165b during ovarian follicular development
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Abstract
Objective: To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles. Methods: We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence. Results: The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h. Conclusions: This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.