Determination of T Cell Responses in Thai Systemic Sclerosis Patients
Issued Date
2022-01-01
Resource Type
ISSN
23148861
eISSN
23147156
Scopus ID
2-s2.0-85126866621
Pubmed ID
35310606
Journal Title
Journal of Immunology Research
Volume
2022
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Immunology Research Vol.2022 (2022)
Suggested Citation
Likhit O., Louthrenoo W., Pattanakitsakul S.N., Suttitheptumrong A., Hannongbua S., Rungrotmongkol T., Noguchi H., Takeuchi F., Boonnak K. Determination of T Cell Responses in Thai Systemic Sclerosis Patients. Journal of Immunology Research Vol.2022 (2022). doi:10.1155/2022/5072154 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/85056
Title
Determination of T Cell Responses in Thai Systemic Sclerosis Patients
Other Contributor(s)
Abstract
Objectives. This study is aimed at determining the role of T cells by assessing the numbers of IFN-γ- and IL-2-secreting T cells following stimulation with peptides derived from DNA topoisomerase-I protein in Thai SSc patients. Methods. Fifty Thai SSc patients and 50 healthy controls (HC) joined this study. IFN-γ and IL-2 levels upon stimulation of T cells with 6 peptides derived from DNA topoisomerase-I protein were determined. Anti-nuclear antibodies (ANA) and anti-Scl-70 antibodies were determined by using the ELISA method. Results. In SSc patients, we detected a significantly higher number of IFN-γ- and IL-2-secreting CD8+ T cells than IFN-γ- and IL-2-secreting CD4+ T cells after stimulation with pooled peptides derived from DNA topoisomerase-I protein. A similar percentage of CD4+IL-2+, CD4+IFN-γ+, and CD8+IL-2+ were detected following stimulation with DNA topoisomerase-I protein -in SSc patients with anti-Scl-70 antibody (SSc/anti-Scl-70+) and those without. In contrast, the amount of CD8+IFN-γ+ cells was significantly higher in SSc/anti-Scl-70+ than those without. Stimulation with individual peptides showed that CSLRVEHINLHPELD (sPep3; 15 amino acids; position 505-519 of DNA topoisomerase-I protein) was the optimal epitope that induced T cells secreting the highest levels of IFN-γ and IL-2. A higher percentage of IFN-γ+CD4+ T cells was detected in SSc/anti-Scl-70+ than those without the following stimulation with peptides 2 (amino acid position 475-486 [RAVALYFIDKLA] of protein DNA topoisomerase). Conclusion. The results from this study emphasize the critical role of DNA topoisomerase-I peptides on the activation of T cells in SSc patients. The findings provide a better understanding of SSc's immunopathogenesis and may lead to the development of diagnostic tools and specific treatments for SSc in the future.