Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth
Issued Date
2024-06-01
Resource Type
ISSN
14326981
eISSN
14363771
Scopus ID
2-s2.0-85193484000
Pubmed ID
38758416
Journal Title
Clinical Oral Investigations
Volume
28
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Clinical Oral Investigations Vol.28 No.6 (2024)
Suggested Citation
Lertruangpanya K., Roytrakul S., Surarit R., Horsophonphong S. Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth. Clinical Oral Investigations Vol.28 No.6 (2024). doi:10.1007/s00784-024-05698-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98473
Title
Comparative proteomic analysis of dental pulp from supernumerary and normal permanent teeth
Corresponding Author(s)
Other Contributor(s)
Abstract
Objectives: To obtain and compare the protein profiles of supernumerary and normal permanent dental pulp tissues. Materials and methods: Dental pulp tissues were obtained from supernumerary and normal permanent teeth. Proteins were extracted and analyzed by liquid chromatography-tandem mass spectrometry (LC/MS-MS). Protein identification and quantification from MS data was performed with MaxQuant. Statistical analysis was conducted using Metaboanalyst to identify differentially expressed proteins (DEPs) (P-value < 0.05, fold-change > 2). Gene Ontology enrichment analyses were performed with gProfiler. Results: A total of 3,534 proteins were found in normal dental pulp tissue and 1,093 in supernumerary dental pulp tissue, with 174 DEPs between the two groups. This analysis revealed similar functional characteristics in terms of cellular component organization, cell differentiation, developmental process, and response to stimulus, alongside exclusive functions unique to normal permanent dental pulp tissues such as healing, vascular development and cell death. Upon examination of DEPs, these proteins were associated with the processes of wound healing and apoptosis. Conclusions: This study provides a comprehensive understanding of the protein profile of dental pulp tissue, including the first such profiling of supernumerary permanent dental pulp. There are functional differences between the proteomic profiles of supernumerary and normal permanent dental pulp tissue, despite certain biological similarities between the two groups. Differences in protein expression were identified, and the identified DEPs were linked to the healing and apoptosis processes. Clinical relevance: This discovery enhances our knowledge of supernumerary and normal permanent pulp tissue, and serves as a valuable reference for future studies on supernumerary teeth.