Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
Issued Date
2022-01-01
Resource Type
ISSN
10643745
eISSN
19406029
Scopus ID
2-s2.0-85129999197
Pubmed ID
35524112
Journal Title
Methods in Molecular Biology
Volume
2477
Start Page
71
End Page
77
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in Molecular Biology Vol.2477 (2022) , 71-77
Suggested Citation
Wongsurawat T., Jenjaroenpun P., Nookaew I. Direct Sequencing of RNA and RNA Modification Identification Using Nanopore. Methods in Molecular Biology Vol.2477 (2022) , 71-77. 77. doi:10.1007/978-1-0716-2257-5_5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83911
Title
Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA.