Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation
Issued Date
2022-04-01
Resource Type
eISSN
20734409
Scopus ID
2-s2.0-85126929959
Pubmed ID
35406667
Journal Title
Cells
Volume
11
Issue
7
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cells Vol.11 No.7 (2022)
Suggested Citation
Saithong S., Worasilchai N., Saisorn W., Udompornpitak K., Bhunyakarnjanarat T., Chindamporn A., Tovichayathamrong P., Torvorapanit P., Chiewchengchol D., Chancharoenthana W., Leelahavanichkul A. Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation. Cells Vol.11 No.7 (2022). doi:10.3390/cells11071103 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83783
Title
Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation
Other Contributor(s)
Abstract
Due to limited data on the link between gut barrier defects (leaky gut) and neutrophil extracellular traps (NETs) in coronavirus disease 2019 (COVID-19), blood samples of COVID-19 cases—mild (upper respiratory tract symptoms without pneumonia; n = 27), moderate (pneumonia without hypoxia; n = 28), and severe (pneumonia with hypoxia; n = 20)—versus healthy control (n = 15) were evaluated, together with in vitro experiments. Accordingly, neutrophil counts, serum cytokines (IL-6 and IL-8), lipopolysaccharide (LPS), bacteria-free DNA, and NETs parameters (flu-orescent-stained nuclear morphology, dsDNA, neutrophil elastase, histone–DNA complex, and myeloperoxidase–DNA complex) were found to differentiate COVID-19 severity, whereas serum (1→3)-β-D-glucan (BG) was different between the control and COVID-19 cases. Despite non-detect-able bacteria-free DNA in the blood of healthy volunteers, using blood bacteriome analysis, prote-obacterial DNA was similarly predominant in both control and COVID-19 cases (all severities). In parallel, only COVID-19 samples from moderate and severe cases, but not mild cases, were activated in vitro NETs, as determined by supernatant dsDNA, Peptidyl Arginine Deiminase 4, and nuclear morphology. With neutrophil experiments, LPS plus BG (LPS + BG) more prominently induced NETs, cytokines, NFκB, and reactive oxygen species, when compared with the activation by each molecule alone. In conclusion, pathogen molecules (LPS and BG) from gut translocation along with neutrophilia and cytokinemia in COVID-19-activated, NETs-induced hyperinflammation.