Quantitative Determination of Iron-Siderophore Complexes in Peat by Isotope-Exchange Size-Exclusion UPLC-Electrospray Ionization High-Resolution Accurate Mass (HRAM) Mass Spectrometry
Issued Date
2023-01-01
Resource Type
ISSN
00032700
eISSN
15206882
Scopus ID
2-s2.0-85163720321
Journal Title
Analytical Chemistry
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Chemistry (2023)
Suggested Citation
Maknun L., Kińska K., González-Álvarez I., Ouerdane L., Lauga B., Siripinyanond A., Szpunar J., Lobinski R. Quantitative Determination of Iron-Siderophore Complexes in Peat by Isotope-Exchange Size-Exclusion UPLC-Electrospray Ionization High-Resolution Accurate Mass (HRAM) Mass Spectrometry. Analytical Chemistry (2023). doi:10.1021/acs.analchem.3c00122 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87987
Title
Quantitative Determination of Iron-Siderophore Complexes in Peat by Isotope-Exchange Size-Exclusion UPLC-Electrospray Ionization High-Resolution Accurate Mass (HRAM) Mass Spectrometry
Author's Affiliation
Other Contributor(s)
Abstract
A method was developed for the quantification of iron-siderophore complexes by electrospray high-resolution accurate mass (HRAM) mass spectrometry (MS) without the need for authentic standards. The bulk of iron-siderophore complexes was purified by solid-phase extraction (SPE) and concentrated by evaporation. The individual complexes were identified by fast size-exclusion chromatography (FastSEC)-Orbitrap MSn on the basis of the exact molecular mass (±1 ppm) and MS2 or MS3 fragmentation. Their capability to readily exchange the natural 56Fe for the added 58Fe was demonstrated by SEC with ICP MS and ESI MS detection. The method was applied to the analysis of peat sampled in the eastern part of the French Pyrenean mountains. Nineteen siderophores belonging to four different classes were identified and quantified. The results were validated using ICP MS detection of iron by matching the sum of iron complexes determined by isotope exchange-ESI MS within each peak observed by FastSEC-ICP MS.