A Multianalyte Electrochemical Genosensor for the Detection of High-Risk HPV Genotypes in Oral and Cervical Cancers
Issued Date
2022-05-01
Resource Type
eISSN
20796374
Scopus ID
2-s2.0-85130157846
Pubmed ID
35624591
Journal Title
Biosensors
Volume
12
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biosensors Vol.12 No.5 (2022)
Suggested Citation
Chaibun T., Thanasapburachot P., Chatchawal P., Yin L.S., Jiaranuchart S., Jearanaikoon P., Promptmas C., Buajeeb W., Lertanantawong B. A Multianalyte Electrochemical Genosensor for the Detection of High-Risk HPV Genotypes in Oral and Cervical Cancers. Biosensors Vol.12 No.5 (2022). doi:10.3390/bios12050290 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83745
Title
A Multianalyte Electrochemical Genosensor for the Detection of High-Risk HPV Genotypes in Oral and Cervical Cancers
Other Contributor(s)
Abstract
Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. Hence, we developed a multianalyte electrochemical DNA biosensor that could be used for both oral and cervical samples to detect the high-risk HPV genotypes 16 and 18. The assay involves the sandwich hybridization of the HPV target to the silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with a detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM and 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR/gel electrophoresis detection. The biosensor assay could be completed within 90 min. Due to its simplicity, rapidity, and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories as well as for epidemiological studies.