Development and validation of a reversed-phase high performance liquid chromatographic method for simultaneous determination of miconazole and triamcinolone in cream
Issued Date
2016
Copyright Date
2016
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xix, 104 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Pharmaceutical Chemistry and Phytochemistry))--Mahidol University, 2016
Suggested Citation
Zin, Zin Ei, 1984- Development and validation of a reversed-phase high performance liquid chromatographic method for simultaneous determination of miconazole and triamcinolone in cream. Thesis (M.Sc. (Pharmaceutical Chemistry and Phytochemistry))--Mahidol University, 2016. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/93275
Title
Development and validation of a reversed-phase high performance liquid chromatographic method for simultaneous determination of miconazole and triamcinolone in cream
Author(s)
Abstract
Miconazole (MIC) and triamcinolone (TRI) are used as a combination in formulation of topical cream for their antifungal infection and anti-inflammatory action. In this study, a simple, precise and accurate reversed-phase high performance liquid chromatography method was developed for the simultaneous determination of MIC, TRI, methylparaben (MEP), and propylparaben (PRP) in cream by using finasteride as the internal standard. The complete separation of the compounds was achieved on the C8 column (150 x 4.6 mm, 5 µm, particle size) with isocratic elution by using the mobile phase mixture of 5 mM trichloroacetic acid in 0.05 % phosphoric acid and acetonitrile (52 : 48 % v/v). The mobile phase flow rate was set to 0.9 mL/min. The UV detector was used at 264 nm. All target compounds were separated within 8.0 min. Analytical performance of the developed HPLC procedure was validated for system suitability, accuracy, intermediate precision, repeatability, specificity, linearity and range according to ICH guideline. The percent recoveries were 100.7-104.6 % for MIC and 98.2-99.6 % for TRI. Repeatability and intermediate precision, presented as % RSD values, were less than 6.1 and 1.8, respectively. The specificity results indicated that the determination of MIC and TRI in cream was performed without interferences from other excipients. The linear regression analysis for the calibration curve of MIC and TRI showed a linear relationship with the square of correlation coefficient (r) value of MIC and TRI, 0.9988 and 0.9982, respectively, in the concentration range of 0.05 to 0.5 mg/mL for MIC and 0.005 to 0.05 mg/mL for TRI. The percent labeled amount for quantitative determination of MIC and TRI in four cream formulations from the market were 103.8 ± 2.1, 101.5 ± 1.1, 99.4 ± 1.4, 100.3 ± 1.3 for MIC and 104.7 ± 2.1, 101.3 ± 1.0, 114.2 ± 1.3, 117.7± 1.5 for TRI, respectively.
Description
Pharmaceutical Chemistry and Phytochemistry (Mahidol University 2016)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Pharmacy
Degree Discipline
Pharmaceutical Chemistry and Phytochemistry
Degree Grantor(s)
Mahidol University