Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy
Issued Date
2022-10-01
Resource Type
ISSN
01054538
eISSN
13989995
Scopus ID
2-s2.0-85130595458
Pubmed ID
35567339
Journal Title
Allergy: European Journal of Allergy and Clinical Immunology
Volume
77
Issue
10
Start Page
3041
End Page
3051
Rights Holder(s)
SCOPUS
Bibliographic Citation
Allergy: European Journal of Allergy and Clinical Immunology Vol.77 No.10 (2022) , 3041-3051
Suggested Citation
Wai C.Y.Y., Leung N.Y.H., Leung A.S.Y., Ngai S.M., Pacharn P., Yau Y.S., Rosa Duque J.S.D., Kwan M. ., Jirapongsananuruk O., Chan W.H., Chua G., Lee Q.U., Piboonpocanun S., Ho P.K., Wong J.C., Li S., Xu K.J.Y., Wong G.W.K., Chu K., Leung P.S.C., Vichyanond P., Leung T.F. Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy. Allergy: European Journal of Allergy and Clinical Immunology Vol.77 No.10 (2022) , 3041-3051. 3051. doi:10.1111/all.15370 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84903
Title
Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy
Author's Affiliation
Siriraj Hospital
The University of Hong Kong Li Ka Shing Faculty of Medicine
Prince of Wales Hospital Hong Kong
University of California, Davis
Queen Elizabeth Hospital Hong Kong
Princess Margaret Hospital Hong Kong
Institute of Molecular Biosciences, Mahidol University
Chinese University of Hong Kong
Samitivej Thonburi Hospital
The University of Hong Kong Li Ka Shing Faculty of Medicine
Prince of Wales Hospital Hong Kong
University of California, Davis
Queen Elizabeth Hospital Hong Kong
Princess Margaret Hospital Hong Kong
Institute of Molecular Biosciences, Mahidol University
Chinese University of Hong Kong
Samitivej Thonburi Hospital
Other Contributor(s)
Abstract
Background: Clinical management of shrimp allergy is hampered by the lack of accurate tests. Molecular diagnosis has been shown to more accurately reflect the clinical reactivity but the full spectrum of shrimp allergens and their clinical relevance are yet to be established. We therefore sought to comprehend the allergen repertoire of shrimp, investigate and compare the sensitization pattern and diagnostic value of the allergens in allergic subjects of two distinct populations. Methods: Sera were collected from 85 subjects with challenge-proven or doctor-diagnosed shrimp allergy in Hong Kong and Thailand. The IgE-binding proteins of Penaeus monodon were probed by Western blotting and identified by mass spectrometry. Recombinant shrimp allergens were synthesized and analyzed for IgE sensitization by ELISA. Results: Ten IgE-binding proteins were identified, and a comprehensive panel of 11 recombinant shrimp allergens was generated. The major shrimp allergens among Hong Kong subjects were troponin C (Pen m 6) and glycogen phosphorylase (Pen m 14, 47.1%), tropomyosin (Pen m 1, 41.2%) and sarcoplasmic-calcium binding protein (Pen m 4, 35.3%), while those among Thai subjects were Pen m 1 (68.8%), Pen m 6 (50.0%) and fatty acid-binding protein (Pen m 13, 37.5%). Component-based tests yielded significantly higher area under curve values (0.77–0.96) than shrimp extract-IgE test (0.70–0.75). Yet the best component test differed between populations; Pen m 1-IgE test added diagnostic value only in the Thai cohort, whereas sensitizations to other components were better predictors of shrimp allergy in Hong Kong patients. Conclusion: Pen m 14 was identified as a novel shrimp allergen predictive of challenge outcome. Molecular diagnosis better predicts shrimp allergy than conventional tests, but the relevant component is population dependent.