Rapid On-Site Detection of Hepatitis A and Norovirus in Fresh Food Using Duplex RT-RPA and Lateral Flow Assay
1
Issued Date
2026-03-01
Resource Type
eISSN
27740226
Scopus ID
2-s2.0-105029275372
Journal Title
Trends in Sciences
Volume
23
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Trends in Sciences Vol.23 No.3 (2026)
Suggested Citation
Intamaso U., Chutoam P., Jirasatid S., Wiwekwin N., Saimuang K., Lertjuthaporn S., Kaewdum M., Jangpatarapongsa K. Rapid On-Site Detection of Hepatitis A and Norovirus in Fresh Food Using Duplex RT-RPA and Lateral Flow Assay. Trends in Sciences Vol.23 No.3 (2026). doi:10.48048/tis.2026.11645 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114963
Title
Rapid On-Site Detection of Hepatitis A and Norovirus in Fresh Food Using Duplex RT-RPA and Lateral Flow Assay
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Corresponding Author(s)
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Abstract
Foodborne viruses, such as hepatitis A virus (HAV) and norovirus (NoV), are major public health threats, highlighting the need for rapid, affordable, and user-friendly detection methods. This study introduces a new method that simplifies food extraction and uses duplex RT-RPA combined with a lateral flow assay (LFA) to detect HAV and NoV simultaneously in fresh foods like fresh shellfish, meats, and produce. The RT-RPA conditions and LFA strip design were optimized to improve sensitivity and accuracy. The method was validated for cross-reactivity, sensitivity, and accuracy through interlaboratory testing. It achieved a detection limit of 10<sup>4</sup>RNA copies per reaction in purified RNA for both HAV and NoV, with no cross-reactivity observed. Among 200 fresh food samples tested, 76.5% (153/200) were contaminated, and 45% (90/200) showed co-contamination with HAV and NoV. Additionally, blind testing by a non-expert achieved 96% accuracy. Compared to existing molecular methods, the main advantages of RT-RPA over RT-PCR and RT-LAMP are its speed, low-temperature operation, robustness and simplicity. Combining RT-RPA with LFA offers a practical and efficient solution for on-site detection of foodborne viruses, with the potential to enhance food safety and reduce virus transmission.