Mathurameha ameliorates cardiovascular complications in high-fat diet/low-dose streptozotocin-induced type 2 diabetic rats: insights from histological and proteomic analysis
Issued Date
2024-01-01
Resource Type
ISSN
15672379
eISSN
15672387
Scopus ID
2-s2.0-85203016622
Journal Title
Journal of Molecular Histology
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Molecular Histology (2024)
Suggested Citation
Somsuan K., Aluksanasuwan S., Woottisin S., Chiangjong W., Wanta A., Munkong N., Jaidee W., Praman S., Fuangfoo K., Morchang A., Kamsrijai U., Woottisin N., Rujanapun N., Charoensup R. Mathurameha ameliorates cardiovascular complications in high-fat diet/low-dose streptozotocin-induced type 2 diabetic rats: insights from histological and proteomic analysis. Journal of Molecular Histology (2024). doi:10.1007/s10735-024-10258-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101135
Title
Mathurameha ameliorates cardiovascular complications in high-fat diet/low-dose streptozotocin-induced type 2 diabetic rats: insights from histological and proteomic analysis
Corresponding Author(s)
Other Contributor(s)
Abstract
Type 2 diabetes mellitus (T2DM) is a global health concern with increasing prevalence. Mathurameha, a Thai herbal formula, has shown promising glucose-lowering effects and positive impacts on biochemical profiles in diabetic rats. The present study investigated the protective effects of Mathurameha on cardiovascular complications in high-fat diet/streptozotocin (HFD/STZ)-induced type 2 diabetic rats using histological and proteomic analyses. Thirty-five male Sprague-Dawley rats were divided into seven groups: normal diet (ND), ND with aqueous extract (ND + AE450), ND with ethanolic extract (ND + EE200), diabetes (DM), DM with AE (DM + AE450), DM with EE (DM + EE200), and DM with metformin (DM + Met). Mathurameha, especially at 200 mg/kg EE, significantly reduced adipocyte size, cardiac and vascular abnormalities, collagen deposition, and arterial wall thickness in DM rats. Proteomic analysis of rat aortas revealed 30 significantly altered proteins among the ND, DM, and DM + EE200 groups. These altered proteins are involved in various biological processes related to diabetes. Biochemical assays showed that Mathurameha reduced lipid peroxidation (MDA), increased antioxidant levels (GSH), and decreased the expression of inflammatory markers (ICAM1, TNF-α). In conclusion, Mathurameha exhibited significant protective effects against cardiovascular complications in HFD/STZ-induced type 2 diabetic rats through its antioxidant and anti-inflammatory properties.