COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities
Issued Date
2023-07-21
Resource Type
eISSN
25890042
Scopus ID
2-s2.0-85163174183
Journal Title
iScience
Volume
26
Issue
7
Rights Holder(s)
SCOPUS
Bibliographic Citation
iScience Vol.26 No.7 (2023)
Suggested Citation
Wannigama D.L., Amarasiri M., Hongsing P., Hurst C., Modchang C., Chadsuthi S., Anupong S., Phattharapornjaroen P., Ali A.H., Fernandez S., Huang A.T., Vatanaprasan P., Jay D.J., Saethang T., Luk-in S., Storer R.J., Ounjai P., Devanga Ragupathi N.K., Kanthawee P., Sano D., Furukawa T., Sei K., Leelahavanichkul A., Kanjanabuch T., Hirankarn N., Higgins P.G., Kicic A., Singer A.C., Chatsuwan T., Trowsdale S., Abe S., McLellan A.D., Ishikawa H. COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities. iScience Vol.26 No.7 (2023). doi:10.1016/j.isci.2023.107019 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87829
Title
COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities
Author(s)
Wannigama D.L.
Amarasiri M.
Hongsing P.
Hurst C.
Modchang C.
Chadsuthi S.
Anupong S.
Phattharapornjaroen P.
Ali A.H.
Fernandez S.
Huang A.T.
Vatanaprasan P.
Jay D.J.
Saethang T.
Luk-in S.
Storer R.J.
Ounjai P.
Devanga Ragupathi N.K.
Kanthawee P.
Sano D.
Furukawa T.
Sei K.
Leelahavanichkul A.
Kanjanabuch T.
Hirankarn N.
Higgins P.G.
Kicic A.
Singer A.C.
Chatsuwan T.
Trowsdale S.
Abe S.
McLellan A.D.
Ishikawa H.
Amarasiri M.
Hongsing P.
Hurst C.
Modchang C.
Chadsuthi S.
Anupong S.
Phattharapornjaroen P.
Ali A.H.
Fernandez S.
Huang A.T.
Vatanaprasan P.
Jay D.J.
Saethang T.
Luk-in S.
Storer R.J.
Ounjai P.
Devanga Ragupathi N.K.
Kanthawee P.
Sano D.
Furukawa T.
Sei K.
Leelahavanichkul A.
Kanjanabuch T.
Hirankarn N.
Higgins P.G.
Kicic A.
Singer A.C.
Chatsuwan T.
Trowsdale S.
Abe S.
McLellan A.D.
Ishikawa H.
Author's Affiliation
Yamagata Prefectural Central Hospital
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Yamagata Prefectural University of Health Sciences
Kitasato University Graduate School of Medical Science
Perth Children's Hospital
Curtin University
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Naresuan University
King Chulalongkorn Memorial Hospital
Kasetsart University
Mae Fah Luang University
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Tohoku University
The University of Auckland
UK Centre for Ecology & Hydrology
Charles Darwin University
Faculty of Medicine, Chulalongkorn University
Ministry of Higher Education, Science, Research and Innovation
The University of Sheffield
Christian Medical College, Vellore
Telethon Kids Institute
Partner Site Bonn-Cologne
MHESI
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Yamagata Prefectural University of Health Sciences
Kitasato University Graduate School of Medical Science
Perth Children's Hospital
Curtin University
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Naresuan University
King Chulalongkorn Memorial Hospital
Kasetsart University
Mae Fah Luang University
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Tohoku University
The University of Auckland
UK Centre for Ecology & Hydrology
Charles Darwin University
Faculty of Medicine, Chulalongkorn University
Ministry of Higher Education, Science, Research and Innovation
The University of Sheffield
Christian Medical College, Vellore
Telethon Kids Institute
Partner Site Bonn-Cologne
MHESI
Other Contributor(s)
Abstract
Equitable SARS-CoV-2 surveillance in low-resource communities lacking centralized sewers is critical as wastewater-based epidemiology (WBE) progresses. However, large-scale studies on SARS-CoV-2 detection in wastewater from low-and middle-income countries is limited because of economic and technical reasons. In this study, wastewater samples were collected twice a month from 186 urban and rural subdistricts in nine provinces of Thailand mostly having decentralized and non-sewered sanitation infrastructure and analyzed for SARS-CoV-2 RNA variants using allele-specific RT-qPCR. Wastewater SARS-CoV-2 RNA concentration was used to estimate the real-time incidence and time-varying effective reproduction number (Re). Results showed an increase in SARS-CoV-2 RNA concentrations in wastewater from urban and rural areas 14–20 days earlier than infected individuals were officially reported. It also showed that community/food markets were “hot spots” for infected people. This approach offers an opportunity for early detection of transmission surges, allowing preparedness and potentially mitigating significant outbreaks at both spatial and temporal scales.