Comparative analysis of SARS-CoV-2 neutralization titers reveals consistency between human and animal model serum and across assays
Issued Date
2024-05-15
Resource Type
eISSN
19466242
Scopus ID
2-s2.0-85193357220
Pubmed ID
38748773
Journal Title
Science translational medicine
Volume
16
Issue
747
Rights Holder(s)
SCOPUS
Bibliographic Citation
Science translational medicine Vol.16 No.747 (2024) , eadl1722
Suggested Citation
Mühlemann B., Wilks S.H., Baracco L., Bekliz M., Carreño J.M., Corman V.M., Davis-Gardner M.E., Dejnirattisai W., Diamond M.S., Douek D.C., Drosten C., Eckerle I., Edara V.V., Ellis M., Fouchier R.A.M., Frieman M., Godbole S., Haagmans B., Halfmann P.J., Henry A.R., Jones T.C., Katzelnick L.C., Kawaoka Y., Kimpel J., Krammer F., Lai L., Liu C., Lusvarghi S., Meyer B., Mongkolsapaya J., Montefiori D.C., Mykytyn A., Netzl A., Pollett S., Rössler A., Screaton G.R., Shen X., Sigal A., Simon V., Subramanian R., Supasa P., Suthar M.S., Türeli S., Wang W., Weiss C.D., Smith D.J. Comparative analysis of SARS-CoV-2 neutralization titers reveals consistency between human and animal model serum and across assays. Science translational medicine Vol.16 No.747 (2024) , eadl1722. doi:10.1126/scitranslmed.adl1722 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98456
Title
Comparative analysis of SARS-CoV-2 neutralization titers reveals consistency between human and animal model serum and across assays
Author(s)
Mühlemann B.
Wilks S.H.
Baracco L.
Bekliz M.
Carreño J.M.
Corman V.M.
Davis-Gardner M.E.
Dejnirattisai W.
Diamond M.S.
Douek D.C.
Drosten C.
Eckerle I.
Edara V.V.
Ellis M.
Fouchier R.A.M.
Frieman M.
Godbole S.
Haagmans B.
Halfmann P.J.
Henry A.R.
Jones T.C.
Katzelnick L.C.
Kawaoka Y.
Kimpel J.
Krammer F.
Lai L.
Liu C.
Lusvarghi S.
Meyer B.
Mongkolsapaya J.
Montefiori D.C.
Mykytyn A.
Netzl A.
Pollett S.
Rössler A.
Screaton G.R.
Shen X.
Sigal A.
Simon V.
Subramanian R.
Supasa P.
Suthar M.S.
Türeli S.
Wang W.
Weiss C.D.
Smith D.J.
Wilks S.H.
Baracco L.
Bekliz M.
Carreño J.M.
Corman V.M.
Davis-Gardner M.E.
Dejnirattisai W.
Diamond M.S.
Douek D.C.
Drosten C.
Eckerle I.
Edara V.V.
Ellis M.
Fouchier R.A.M.
Frieman M.
Godbole S.
Haagmans B.
Halfmann P.J.
Henry A.R.
Jones T.C.
Katzelnick L.C.
Kawaoka Y.
Kimpel J.
Krammer F.
Lai L.
Liu C.
Lusvarghi S.
Meyer B.
Mongkolsapaya J.
Montefiori D.C.
Mykytyn A.
Netzl A.
Pollett S.
Rössler A.
Screaton G.R.
Shen X.
Sigal A.
Simon V.
Subramanian R.
Supasa P.
Suthar M.S.
Türeli S.
Wang W.
Weiss C.D.
Smith D.J.
Author's Affiliation
F. Edward Hebert School of Medicine
Siriraj Hospital
Oxford University Hospitals NHS Foundation Trust
Africa Health Research Institute
Berliner Institut für Gesundheitsforschung
The Institute of Medical Science, The University of Tokyo
Centre for the AIDS Programme of Research in South Africa
Erasmus MC
University of Cambridge
University of Oxford
Université de Genève Faculté de Médecine
Charité – Universitätsmedizin Berlin
The University of Tokyo
University of Wisconsin School of Veterinary Medicine
National Center for Global Health and Medicine
Washington University School of Medicine in St. Louis
National Institute of Allergy and Infectious Diseases (NIAID)
HJF
Icahn School of Medicine at Mount Sinai
University of Maryland School of Medicine
University of KwaZulu-Natal
Food and Drug Administration
Hôpitaux Universitaires de Genève
Nuffield Department of Medicine
Medizinische Universitat Innsbruck
Duke University School of Medicine
Université de Genève
Emory University School of Medicine
Siriraj Hospital
Oxford University Hospitals NHS Foundation Trust
Africa Health Research Institute
Berliner Institut für Gesundheitsforschung
The Institute of Medical Science, The University of Tokyo
Centre for the AIDS Programme of Research in South Africa
Erasmus MC
University of Cambridge
University of Oxford
Université de Genève Faculté de Médecine
Charité – Universitätsmedizin Berlin
The University of Tokyo
University of Wisconsin School of Veterinary Medicine
National Center for Global Health and Medicine
Washington University School of Medicine in St. Louis
National Institute of Allergy and Infectious Diseases (NIAID)
HJF
Icahn School of Medicine at Mount Sinai
University of Maryland School of Medicine
University of KwaZulu-Natal
Food and Drug Administration
Hôpitaux Universitaires de Genève
Nuffield Department of Medicine
Medizinische Universitat Innsbruck
Duke University School of Medicine
Université de Genève
Emory University School of Medicine
Corresponding Author(s)
Other Contributor(s)
Abstract
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires ongoing monitoring to judge the ability of newly arising variants to escape the immune response. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal serum samples. We compared 18 datasets generated using human, hamster, and mouse serum and six different neutralization assays. Datasets using animal model serum samples showed higher titer magnitudes than datasets using human serum samples in this comparison. Fold change in neutralization of variants compared to ancestral SARS-CoV-2, immunodominance patterns, and antigenic maps were similar among serum samples and assays. Most assays yielded consistent results, except for differences in fold change in cytopathic effect assays. Hamster serum samples were a consistent surrogate for human first-infection serum samples. These results inform the transition of surveillance of SARS-CoV-2 antigenic variation from dependence on human first-infection serum samples to the utilization of serum samples from animal models.