Increased platelet activation and lower platelet-monocyte aggregates in COVID-19 patients with severe pneumonia
Issued Date
2023-03-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85149733480
Pubmed ID
36888618
Journal Title
PLoS ONE
Volume
18
Issue
3 March
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.18 No.3 March (2023)
Suggested Citation
Srihirun S., Sriwantana T., Srichatrapimuk S., Vivithanaporn P., Kirdlarp S., Sungkanuparph S., Phusanti S., Nanthatanti N., Suwannalert P., Sibmooh N. Increased platelet activation and lower platelet-monocyte aggregates in COVID-19 patients with severe pneumonia. PLoS ONE Vol.18 No.3 March (2023). doi:10.1371/journal.pone.0282785 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82146
Title
Increased platelet activation and lower platelet-monocyte aggregates in COVID-19 patients with severe pneumonia
Other Contributor(s)
Abstract
Background The increased procoagulant platelets and platelet activation are associated with thrombosis in COVID-19. In this study, we investigated platelet activation in COVID-19 patients and their association with other disease markers. Methods COVID-19 patients were classified into three severity groups: no pneumonia, mild-to-moderate pneumonia, and severe pneumonia. The expression of P-selectin and activated glycoprotein (aGP) IIb/IIIa on the platelet surface and platelet-leukocyte aggregates were measured prospectively on admission days 1, 7, and 10 by flow cytometry. Results P-selectin expression, platelet-neutrophil, platelet-lymphocyte, and platelet-monocyte aggregates were higher in COVID-19 patients than in uninfected control individuals. In contrast, aGPIIb/IIIa expression was not different between patients and controls. Severe pneumonia patients had lower platelet-monocyte aggregates than patients without pneumonia and patients with mild-to-moderate pneumonia. Platelet-neutrophil and platelet-lymphocyte aggregates were not different among groups. There was no change in platelet-leukocyte aggregates and P-selectin expression on days 1, 7, and 10. aGPIIb/IIIa expression was not different among patient groups. Still, adenosine diphosphate (ADP)-induced aGPIIb/IIIa expression was lower in severe pneumonia than in patients without and with mild-to-moderate pneumonia. Platelet-monocyte aggregates exhibited a weak positive correlation with lymphocyte count and weak negative correlations with interleukin-6, D-dimer, lactate dehydrogenase, and nitrite. Conclusion COVID-19 patients have higher platelet-leukocyte aggregates and P-selectin expression than controls, indicating increased platelet activation. Compared within patient groups, platelet-monocyte aggregates were lower in severe pneumonia patients.