Evaluation of magnetic hyperthermia, drug delivery and biocompatibility (bone cell adhesion and zebrafish assays) of trace element co-doped hydroxyapatite combined with Mn-Zn ferrite for bone tissue applications
Issued Date
2024-09-16
Resource Type
eISSN
20462069
Scopus ID
2-s2.0-85204398706
Journal Title
RSC Advances
Volume
14
Issue
40
Start Page
29242
End Page
29253
Rights Holder(s)
SCOPUS
Bibliographic Citation
RSC Advances Vol.14 No.40 (2024) , 29242-29253
Suggested Citation
Tithito T., Sillapaprayoon S., Chantho V., Pimtong W., Thongbunchoo J., Charoenphandhu N., Krishnamra N., Yong N., Lert-Itthiporn A., Maneeprakorn W., Pon-On W. Evaluation of magnetic hyperthermia, drug delivery and biocompatibility (bone cell adhesion and zebrafish assays) of trace element co-doped hydroxyapatite combined with Mn-Zn ferrite for bone tissue applications. RSC Advances Vol.14 No.40 (2024) , 29242-29253. 29253. doi:10.1039/d4ra03867c Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101365
Title
Evaluation of magnetic hyperthermia, drug delivery and biocompatibility (bone cell adhesion and zebrafish assays) of trace element co-doped hydroxyapatite combined with Mn-Zn ferrite for bone tissue applications
Corresponding Author(s)
Other Contributor(s)
Abstract
The treatment and regeneration of bone defects, especially tumor-induced defects, is an issue in clinical practice and remains a major challenge for bone substitute material invention. In this research, a composite material of biomimetic bone-like apatite based on trace element co-doped apatite (Ca10−δMδ(PO4)5.5(CO3)0.5(OH)2; M = trace elements of Mg, Fe, Zn, Mn, Cu, Ni, Mo, Sr and BO33−; THA)-integrated-biocompatible magnetic Mn-Zn ferrite ((Mn, Zn)Fe2O4 nanoparticles, BioMags) called THAiBioMags was prepared via a co-precipitation method. Its characteristics, i.e., physical properties, hyperthermia performance, ion/drug delivery, were investigated in vitro using osteoblasts (bone-forming cells) and in vivo using zebrafish. The synthesized THAiBioMags particles revealed superparamagnetic behaviour at room temperature. Under the influence of an alternating magnetic field, THAiBioMags particles demonstrated a change in temperature, indicating their potential for magnetic hyperthermia, in which THAiBioMags further exhibited a specific absorption rate (SAR) value of 9.44 W g−1 (I = 44 A, H = 6.03 kA m−1 and f = 130 kHz). In addition, the as-prepared particles demonstrated sustained ion/drug (doxorubicin (DOX)) release under physiological pH conditions. Biological assay analysis revealed that THAiBioMags exhibited no toxicity towards osteoblast cells and demonstrated excellent cell adhesion properties. In vivo studies employing an embryonic zebrafish model showed the non-toxic nature of the synthesized THAiBioMags particles, as revealed by evaluation of the survivability, hatching rate, and embryonic morphology. These results could potentially lead to the design and fabrication of magnetic scaffolds to be used in therapeutic treatment and bone regeneration.