Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells
Issued Date
2024-05-01
Resource Type
ISSN
11217138
Scopus ID
2-s2.0-85192034837
Pubmed ID
38700885
Journal Title
The new microbiologica
Volume
47
Issue
1
Start Page
60
End Page
67
Rights Holder(s)
SCOPUS
Bibliographic Citation
The new microbiologica Vol.47 No.1 (2024) , 60-67
Suggested Citation
Sankuntaw N., Punyadee N., Chantratita W., Lulitanond V. Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells. The new microbiologica Vol.47 No.1 (2024) , 60-67. 67. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98277
Title
Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells
Author(s)
Corresponding Author(s)
Other Contributor(s)
Abstract
Acute respiratory tract infection (ARTI) is common in all age groups, especially in children and the elderly. About 85% of children who present with bronchiolitis are infected with respiratory syncytial virus (RSV); however, nearly one-third are coinfected with another respiratory virus, such as human rhinovirus (HRV). Therefore, it is necessary to explore the immune response to coinfection to better understand the molecular and cellular pathways involving virus-virus interactions that might be modulated by innate immunity and additional host cell response mechanisms. This study aims to investigate the host innate immune response against RSV-HRV coinfection compared with monoinfection. Human primary bronchial/tracheal epithelial cells (HPECs) were infected with RSV, HRV, or coinfected with both viruses, and the infected cells were collected at 48 and 72 hours. Gene expression profiles of IL-6, CCL5, TNF-α, IFN-β, IFN-λ1, CXCL10, IL-10, IL-13, IRF3, and IRF7 were investigated using real-time quantitative PCR, which revealed that RSV-infected cells exhibited increased expression of IL-10, whereas HRV infection increased the expression of CXCL10, IL-10, and CCL5. IFN-λ1 and CXCL10 expression was significantly different between the coinfection and monoinfection groups. In conclusion, our study revealed that two important cytokines, IFN-λ1 and CXCL10, exhibited increased expression during coinfection.