In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats
Issued Date
2022-06-01
Resource Type
eISSN
23048158
Scopus ID
2-s2.0-85131552578
Journal Title
Foods
Volume
11
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Foods Vol.11 No.11 (2022)
Suggested Citation
Jaroenporn C., Supawasit W., Bundidamorn D., Udompijitkul P., Assawamakin A., Trevanich S. In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats. Foods Vol.11 No.11 (2022). doi:10.3390/foods11111557 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83220
Title
In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats
Author's Affiliation
Other Contributor(s)
Abstract
The aim of the study was to perform in-house validation of the developed multiplex PCR (mPCR)-based alternative method to detect Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw meats following the ISO 16140-2: 2016. A comparative study of the developed mPCR against the Bacteriological Analytical Manual (BAM) method was evaluated for inclusivity and exclusivity, sensitivity and the relative level of detection (RLOD). Inclusivity levels for each target bacterium were all 100%, while exclusivity for non-target bacteria was 100%. The sensitivity of the developed mPCR was calculated based on the analysis of 72 samples of raw meat. The sensitivity of the developed mPCR was 100%. The RLOD values of the developed mPCR for STEC, L. monocytogenes and Salmonella spp. were 0.756, 1.170 and 1.000, respectively. The developed mPCR showed potential as a tool for the fast, specific and sensitive detection of the three bacteria in the raw meat industry.