Revealing holistic metabolic responses associated with lipid and docosahexaenoic acid (DHA) production in Aurantiochytrium sp. SW1
Issued Date
2023-05-01
Resource Type
ISSN
13881981
eISSN
18792618
Scopus ID
2-s2.0-85149853609
Pubmed ID
36907245
Journal Title
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume
1868
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids Vol.1868 No.5 (2023)
Suggested Citation
Prabhakaran P., Raethong N., Thananusak R., Nazir M.Y.M., Sapkaew C., Soommat P., Kingkaw A., Hamid A.A., Vongsangnak W., Song Y. Revealing holistic metabolic responses associated with lipid and docosahexaenoic acid (DHA) production in Aurantiochytrium sp. SW1. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids Vol.1868 No.5 (2023). doi:10.1016/j.bbalip.2023.159306 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81598
Title
Revealing holistic metabolic responses associated with lipid and docosahexaenoic acid (DHA) production in Aurantiochytrium sp. SW1
Other Contributor(s)
Abstract
Aurantiochytrium sp. SW1, a marine thraustochytrid, has been regarded as a potential candidate as a docosahexaenoic acid (DHA) producer. Even though the genomics of Aurantiochytrium sp. are available, the metabolic responses at a systems level are largely unknown. Therefore, this study aimed to investigate the global metabolic responses to DHA production in Aurantiochytrium sp. through transcriptome and genome-scale network-driven analysis. Of a total of 13,505 genes, 2527 differentially expressed genes (DEGs) were identified in Aurantiochytrium sp., unravelling the transcriptional regulations behinds lipid and DHA accumulation. The highest number of DEG were found for pairwise comparison between growth phase and lipid accumulating phase where a total of 1435 genes were down-regulated with 869 genes being up-regulated. These uncovered several metabolic pathways that contributing in DHA and lipid accumulation including amino acid and acetate metabolism which involve in the generation of crucial precursors. Upon applying network-driven analysis, hydrogen sulphide was found as potential reporter metabolite that could be associated with the genes related to acetyl-CoA synthesis for DHA production. Our findings suggest that the transcriptional regulation of these pathways is a ubiquitous feature in response to specific cultivation phases during DHA overproduction in Aurantiochytrium sp. SW1.