UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors
Issued Date
2022-01-15
Resource Type
ISSN
13858947
Scopus ID
2-s2.0-85115632235
Journal Title
Chemical Engineering Journal
Volume
428
Rights Holder(s)
SCOPUS
Bibliographic Citation
Chemical Engineering Journal Vol.428 (2022)
Suggested Citation
Khunkitchai N., Nuchtavorn N., Rypar T., Vlcnovska M., Nejdl L., Vaculovicova M., Macka M. UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors. Chemical Engineering Journal Vol.428 (2022). doi:10.1016/j.cej.2021.132508 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84213
Title
UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors
Other Contributor(s)
Abstract
Quantum dots (QDs) have been widely applied in the analytical field including sensitive fluorescent assays on paper-based devices. A facile in-situ synthesis of QDs after a simple reagent deposition on paper could enable a rapid low-cost fabrication of QD–modified paper with a variety of properties and purposes such as enzymatic nanoreactors. Herein, for the first time, fluorescent ZnCd QDs were prepared by in-situ synthesis in the paper matrix using thiol-containing precursors and irradiation by UV light. A successful creation of the immobilized ZnCd QDs on paper (paper@ZnCd QDs) was monitored by their intrinsic fluorescence while their peroxidase mimetic activity was evaluated by a catalytic reaction between H2O2 and a substrate (3,3′,5,5′-tetramethylbenzidine, TMB) producing a blue coloured product of charged transfer complex of TMB (oxTMB). From three thiol precursors investigated, the insulin precursor provided a greater activity than glutathione (GSH) and both considerably larger than BSA. Finally, a QDs precursor mixture deposition onto the paper matrix was evaluated by the peroxidase-mimetic activity, which was comparable with a reference method. The in-situ preparation of paper@ZnCd QDs is simple, rapid and ‘green’, with potential in biomedical applications primarily as fluorescence imaging agent and enzyme mimetic paper-based nanoreactors.