Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates
Issued Date
2024-01-01
Resource Type
eISSN
19406029
Scopus ID
2-s2.0-85184392543
Pubmed ID
38315364
Journal Title
Methods in molecular biology (Clifton, N.J.)
Volume
2762
Start Page
139
End Page
148
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in molecular biology (Clifton, N.J.) Vol.2762 (2024) , 139-148
Suggested Citation
Urbano-Munoz F., Orne C.E., Burtnick M.N., Brett P.J. Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates. Methods in molecular biology (Clifton, N.J.) Vol.2762 (2024) , 139-148. 148. doi:10.1007/978-1-0716-3666-4_9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97204
Title
Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates
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Abstract
Reductive amination is a relatively simple and convenient strategy for coupling purified polysaccharides to carrier proteins. Following their synthesis, glycoconjugates can be used to assess the protective capacity of specific microbial polysaccharides in animal models of infection and/or to produce polyclonal antiserum and monoclonal antibodies for a variety of immune assays. Here, we describe a reproducible method for chemically activating the 6-deoxyheptan capsular polysaccharide (CPS) from Burkholderia pseudomallei and covalently linking it to recombinant CRM197 diphtheria toxin mutant (CRM197) to produce the glycoconjugate, CPS-CRM197. Similar approaches can also be used to couple other types of polysaccharides to CRM197 with little to no modification of the protocol.