Analysis of Epstein-Barr virus infection in oral potentially malignant disorders and oral cancer: A cross-sectional study
Issued Date
2023-01-01
Resource Type
ISSN
22310762
eISSN
22501002
Scopus ID
2-s2.0-85205286595
Journal Title
Journal of International Society of Preventive and Community Dentistry
Volume
13
Issue
3
Start Page
221
End Page
228
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of International Society of Preventive and Community Dentistry Vol.13 No.3 (2023) , 221-228
Suggested Citation
Pankam J., Lapthanasupkul P., Kitkumthorn N., Rungraungrayabkul D., Klongnoi B., Piboonniyom Khovidhunkit S.O. Analysis of Epstein-Barr virus infection in oral potentially malignant disorders and oral cancer: A cross-sectional study. Journal of International Society of Preventive and Community Dentistry Vol.13 No.3 (2023) , 221-228. 228. doi:10.4103/jispcd.JISPCD_235_22 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102467
Title
Analysis of Epstein-Barr virus infection in oral potentially malignant disorders and oral cancer: A cross-sectional study
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Corresponding Author(s)
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Abstract
Aims and Objectives: The primary objective of this study is to determine the prevalence of Epstein-Barr virus (EBV) in oral potentially malignant disorders (OPMDs) and oral cancer (OC) in a group of Thais using polymerase chain reaction (PCR) and Epstein-Barr encoding regions (EBERs) in situ hybridization (ISH). The secondary objective is to investigate the risk factors of OC and the association between the presence of EBV and risk factors of OC/site of oral lesions. Materials and Methods: Sixty-one participants attending the screening project for OC and OPMDs at the Northeastern district hospitals of Thailand were recruited. Information related to risk factors and biopsy tissues for histopathological diagnosis was collected. Sixty-seven paraffin tissue blocks, including 52 OPMDs and 15 OC specimens, were investigated for EBV infection, using PCR analysis with latent membrane protein-1 (LMP-1) primer and EBERs ISH. Pearson's Chi-square or Fisher's exact test was used to analyze the differences in variables between participants with OPMDs and OC, as appropriate. The association between EBV infection and related risk factors was analyzed using logistic regression with a significant level at 0.05. Results: Using PCR analysis, 8 of 67 specimens (11.94%) were positive for LMP-1. Three cases of OPMDs were positive for both LMP-1 PCR and EBERs ISH. Regarding risk factors of OC, the two most common risk factors were betel nut chewing (52.46%) and working in sunlight (42.62%). The habit of taking alcohol was significantly different between the OC and the OPMDs groups (p = 0.009). The association between LMP-1 and the lesion at the tongue was statistically significant, with odds ratio = 4.900 (95% confidence interval = 1.046-22.943; p = 0.044). Conclusions: The prevalence of EBV infection in this group of participants was low. However, OPMDs at the tongue exhibited a significant association with EBV infection.