Regulation of stress-responsive transcription factors of rice by CPPU, a synthetic cytokinin, during water deficit stress at protein level
Issued Date
2024-01-01
Resource Type
ISSN
01333720
Scopus ID
2-s2.0-85195589456
Journal Title
Cereal Research Communications
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cereal Research Communications (2024)
Suggested Citation
Gujjar R.S., Supaibulwatana K., Srivastava S., Upadhyay A.K. Regulation of stress-responsive transcription factors of rice by CPPU, a synthetic cytokinin, during water deficit stress at protein level. Cereal Research Communications (2024). doi:10.1007/s42976-024-00540-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98820
Title
Regulation of stress-responsive transcription factors of rice by CPPU, a synthetic cytokinin, during water deficit stress at protein level
Corresponding Author(s)
Other Contributor(s)
Abstract
Being one of the major food crops, rice faces enormous yield loss every year due to water scarcity all over the world. To investigate the positive impact of CPPU (N-(2-chloro-4-pyridyl)-N′-phenylurea), a phenylurea cytokinin, on rice plants during drought stress; we planted Oryza sativa ssp. Indica cv. Pathumthani1 under controlled conditions. The plants were subjected to drought stress by withholding water for 14 days (− 70 kPa) at the tillering stage. CPPU was foliar sprayed at 5 mg L−1 on day 6 of drought stress. The influence of CPPU under drought stress, analyzed by nanoLC-MS/MS, revealed 1937 and 1906 differentially expressed proteins in leaf and root tissues, respectively. These differentially expressed proteins were examined for the comparative expression patterns of drought-associated transcription factors in CPPU-treated and untreated plants. The results indicated that CPPU treatment significantly induced the abundance of a large number of transcription factor families including ONAC, MYB, bZIP, ZFP, HD-XIP, HOX, ROC, HSP, HSF, TIFY, WRKY, and AP2/ERF in leaf and root tissues of rice. On the other hand, CPPU treatment also curtailed the expression of many drought/ABA-induced transcription factors like ONAC002, ONAC010-2, ONAC053, ONAC074, ONAC076, MYBAS2, MYB30, GAMYB, bZIP2, bZIP10/ABI5, HD-ZIP HOX8, HD-ZIP HOX9, HD-ZIP TF1, ZFPs (CCCH13, CCCH16, CCCH23, CCCH32, CCCH34, CCCH44, CCCH45, CCCH51, CCCH53, CCCH55, CCCH58, and CCCH67), TIFY2b, WRKY24, WRKY71, and bHLH94 in leaf and root tissues of rice. The results of our study suggest that CPPU helps in moderating the expression of drought-induced transcription factors to sustain normal growth and development in rice plants under water deficit stress.