In vivo culture of Cryptosporidium oocysts for laboratory use
1
Issued Date
2005
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Charoonluk Sangloung, Ruangrat Buddhirongawatr, Nitipan Tantawiwattananon, Yaowalark Sukthana, เยาวลักษณ์ สุขธนะ (2005). In vivo culture of Cryptosporidium oocysts for laboratory use. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63183
Title
In vivo culture of Cryptosporidium oocysts for laboratory use
Other Contributor(s)
Abstract
Cryptosporidium oocysts are needed for laboratory and research work such as drug trials, viability studies and credication methods. Adequately oocysts can be obtained by in vivo and in vitro techniques. We used an in vivo technique in the laboratory. Seven-day-old mice were infected with 100,000-200,000 Cryptosporidium oocysts. At 8 day post infection, the small and large intestine were collected. A simple extraction procedure was used and purification was by Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 °C.
Description
Joint International Tropical Medicine Meeting 2005: The Grand Hotel, Bangkok, Thailand 30 November – 2 December 2005: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2005. p. 173.