Studies on formation, regeneration and fruiting body formation of Pleurotus cystidiosus protoplasts
2
Issued Date
2023
Copyright Date
1991
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 126 leaves : ill. (some col.)
Access Rights
restricted access
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991
Suggested Citation
Sudarat Boonchan Studies on formation, regeneration and fruiting body formation of Pleurotus cystidiosus protoplasts. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90449
Title
Studies on formation, regeneration and fruiting body formation of Pleurotus cystidiosus protoplasts
Alternative Title(s)
การศึกษาการสร้างโปรโตพลาสต์การกลับคืนเป็นเส้นใยและการสร้างดอกเห็ดจากโปรโตพลาสต์ของเห็ดเป๋าฮื้อ
Author(s)
Advisor(s)
Abstract
Conditions suitable for the release of mycelial protoplasts of Pleurotus cystidfosus and for reversion of the isolated protoplasts were examined. The study culminated in the development of a method using commercially available preparation of 2.0 % Novozym(TM) 234, 1.5 % Chitinase, 1.5 % Driselase and 2.0 % Cellulase, by which about 3.8 x 10(6)/ml protoplasts could be obtained from the young growing mycelia, within 10 hours. The 0.05 M Na-maleate buffer containing 0.6 M MgSo(,4), pH 5.8 is suitable as protoplast buffer (buffer/stabilizer). Protoplasts from P. cystidiosus mycelia degenerated into normal hyphae with frequencies about 0.19 % in glucosemalt-yeast extract medium supplemented with 1.0 % sorbitol (regeneration medium), in embedding method. The reversion frequency was shown influence by nutrient composition, especially of carbon and nitrogen sources in the regeneration medium. Mycelium derived from protoplasts of P. cystidiosus consistently developed normal fruiting bodies.
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University