Human Vγ9Vδ2 T cell expansion and their cytotoxic responses against cholangiocarcinoma
Issued Date
2024-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85182158631
Pubmed ID
38221530
Journal Title
Scientific Reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.14 No.1 (2024)
Suggested Citation
Sawaisorn P., Gaballa A., Saimuang K., Leepiyasakulchai C., Lertjuthaporn S., Hongeng S., Uhlin M., Jangpatarapongsa K. Human Vγ9Vδ2 T cell expansion and their cytotoxic responses against cholangiocarcinoma. Scientific Reports Vol.14 No.1 (2024). doi:10.1038/s41598-024-51794-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95760
Title
Human Vγ9Vδ2 T cell expansion and their cytotoxic responses against cholangiocarcinoma
Corresponding Author(s)
Other Contributor(s)
Abstract
Human Vγ9Vδ2 T lymphocytes are regarded as promising effector cells for cancer immunotherapy since they have the ability to eliminate several tumor cells through non-peptide antigen recognition. However, the cytotoxic function and the mechanism of Vγ9Vδ2 T cells leading to specific killing of cholangiocarcinoma cells are yet to be confirmed. In this study, we established a protocol for ex vivo expansion of Vγ9Vδ2 T cells from healthy donors’ peripheral blood mononuclear cells by culture with zoledronate and addition of IL-2, and IL-15 or IL-18 or neither. Testing the cytotoxic capacity of cultured Vγ9Vδ2 T cells against cholangiocarcinoma cell lines showed higher reactivity than against control cells. Surface expression of CD107 was detected on the Vγ9Vδ2 T cells, suggesting that these cells limit in vitro growth of cholangiocarcinoma cells via degranulation of the perforin and granzyme pathway. Analysis of molecular signaling was used to demonstrate expression of pro- and anti-survival genes and a panel of cytokine genes in Vγ9Vδ2 T cells. We found that in the presence of either IL-15 or IL-18, levels of caspase 3 were significantly reduced. Also, IL-15 and IL-18 stimulated cells contained cytotoxicity against cholangiocarcinoma cells, suggesting that stimulated Vγ9Vδ2 T cells may provide a feasible therapy for cholangiocarcinoma.