The relationship between the Glasgow Microenvironment Score and markers of epithelial-mesenchymal transition in TNM II-III colorectal cancer
Issued Date
2022-09-01
Resource Type
ISSN
00468177
eISSN
15328392
Scopus ID
2-s2.0-85132706770
Pubmed ID
35623467
Journal Title
Human Pathology
Volume
127
Start Page
1
End Page
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Human Pathology Vol.127 (2022) , 1-11
Suggested Citation
Alexander P.G., Matly A.A.M., Jirapongwattana N., Pennel K.A.F., van Wyk H.C., McMillan D.C., Horgan P.G., Roxburgh C.S.D., Thuwajit C., Roseweir A.K., Quinn J., Park J.H., Edwards J. The relationship between the Glasgow Microenvironment Score and markers of epithelial-mesenchymal transition in TNM II-III colorectal cancer. Human Pathology Vol.127 (2022) , 1-11. 11. doi:10.1016/j.humpath.2022.05.012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85607
Title
The relationship between the Glasgow Microenvironment Score and markers of epithelial-mesenchymal transition in TNM II-III colorectal cancer
Other Contributor(s)
Abstract
Recently published work on the Glasgow Microenvironment Score (GMS) demonstrated its relevance as a biomarker in TNM II-III colorectal cancer (CRC). Epithelial-mesenchymal transition (EMT) markers in CRC have also shown promise as prognostic biomarkers. This study aimed to assess the relationship between GMS and markers of EMT in stage II-III CRC. A previously constructed tissue microarray of CRC tumors resected between 2000 and 2007 from the Western Infirmary, Stobhill, and Gartnavel General Hospitals in Glasgow was used. Immunohistochemistry was performed for 5 markers of EMT: E-cadherin, β-catenin, Fascin, Snail, and Zeb1. Two-hundred and thirty-eight TNM II-III CRC with valid scores for all EMT markers and GMS were assessed. The prognostic significance of markers of EMT in this cohort and relationships between GMS and markers of EMT were determined. High cytoplasmic and nuclear β-catenin and membrane Zeb-1 were significant for worse cancer-specific survival (hazard ratio [HR] 1.67, 95% confidence interval [CI] 1.01–2.76, P <.05; HR 2.22, 95% CI 1.24–3.97, P <.01; and HR 2.00, 95% CI 1.07–3.77, P =.03, respectively). GMS 0 was associated with low membrane Fascin (P =.03), whereas membrane and cytoplasmic Fascin were observed to be highest in GMS 1, but lower in GMS 2. Nuclear β-catenin was lowest in GMS 0, but highest in GMS 2 (P =.03), in keeping with its role in facilitating EMT. Novel associations were demonstrated between GMS categories and markers of EMT, particularly β-catenin and Fascin, which require further investigation in independent cohorts.