Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives
Issued Date
2023-01-02
Resource Type
ISSN
01681702
eISSN
18727492
Scopus ID
2-s2.0-85142396444
Pubmed ID
36414188
Journal Title
Virus Research
Volume
323
Rights Holder(s)
SCOPUS
Bibliographic Citation
Virus Research Vol.323 (2023)
Suggested Citation
Ruangrung K., Chakritbudsabong W., Thongon S., Rungarunlert S., Wattanapanitch M., Boonarkart C., Suptawiwat O., Sirinonthanawech N., Smith D.R., Auewarakul P. Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives. Virus Research Vol.323 (2023). doi:10.1016/j.virusres.2022.199009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82309
Title
Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives
Other Contributor(s)
Abstract
Influenza A virus (IAV) infection in pregnant women is a major public health concern. However, the effect of IAV infection on human embryogenesis is still unclear. Here we show that human induced pluripotent stem cells (hiPSCs) and hiPSC-derived ectodermal, mesodermal and endodermal cells are susceptible to IAV infection. These cell types stained positive for α2,6-linked sialic acid, the receptor for IAV infection expressed on the cell surface. While hiPSCs produced high viral titers for up to 7 days with increasing infected cell number suggesting that the viral progenies produced from hiPSCs without exogenous protease were infectious and could spread to other cells, the three germ-layer cells showed a decline in viral titers suggesting the lack of viral spreading. Amongst the three germ layers, endodermal cells were less susceptible than ectodermal and mesodermal cells. These results indicate the permissiveness of cells of early embryogenesis, and suggest a risk of detrimental effects of IAV infection in early human embryonic development.