Vacuolating cytotoxin activity and cagA gene of helicobacter pylori Isolates form peptic ulcer and non-ulcer dyspepsia patients from Thailand
Issued Date
2551
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Orasa Suthienkul, อรษา สุตเธียรกุล, Fungfa Uttrarachkij, เฟื่องฟ้า อุตรารัชต์กิจ, Sansanee Tanjatham, ศันสนีย์ ตันติ์จธัม, Siriwat Anantapunpong, สิริวัฒน์ อนันตพันธุ์พงศ์, Suriwan Chantrakooptungool, สุริวรรณ จันทรคุปตังกูร, Suwattana Kanjanahareutai, สุวัฒนา กาญจนหฤทัย (2551). Vacuolating cytotoxin activity and cagA gene of helicobacter pylori Isolates form peptic ulcer and non-ulcer dyspepsia patients from Thailand. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63490
Title
Vacuolating cytotoxin activity and cagA gene of helicobacter pylori Isolates form peptic ulcer and non-ulcer dyspepsia patients from Thailand
Other Contributor(s)
Abstract
The virulence factors of Helicobacter pylori have been associated with clinical presentation and exhibited distinct geographic distribution. In this study, a total of 77 patients were classified at the time of endoscopy as having peptic ulcer diseases (PUD) in 53.2% (41/77), and non-ulcer dyspepsia (NUD) in 46.8% (36/77). All H. pylori tested strains were positive for cagA using PCR. Theprevalence of H. pylori harbouring cytotoxin associated gene A (cagA) in PUD and NUD patients was not significantly diferebt (p>0.05). In addition, 57.7% (41/71) of H. pylori isolates produced vacuolating cytotoxin gene A (VacA) by vacuolization assay in HeLa cell line. No difference in prevalence of VacA producing H. pylori strains was found in PUD (51.5% 24/38) and NUD (63.2%; 17/33) patients (p>0.05). Spearman’s rank correlation test showed no statistical correlation between VacA activity titer and disease status (p>0.05). The results of cagA and VacA in this study were categorized H. pylori into 2 phenotypes; Type 1, ulcerogenic strain (T1U; cagA*VacA*) and Intermediate, nonulcerogenic strain (INU; cagA*VacA*). Of 71 strains, 57.7% and 42.3% represented T1U and INU, respectively. The prevalence of these two types in PUD were T1U, 63.2%, T2NU 236.8% and in NUD were T1U 51.5%, INU48.5%. The prevalence of T1U was not significantly higher in PUD patients than in NUD patients (p>0.05). The results of the analysis indicated that cagA and VacA are not the predictive markers for PUD in our patiens
Description
การประชุมวิชาการสาธารณสุขแห่งชาติ ครั้งที่ 12 คณะสาธารณสุขศาสตร์ มหาวิทยาลัยมหิดล ภาวะโลกร้อนผลกระทบต่อสุขภาพและความรับผิดชอบ: Global warming: health impact and responsibilities, 20-22 สิงหาคม 2551 โรงแรมแอมบาสซาเดอร์ กรุงเทพมหานคร. กรุงเทพฯ: คณะสาธารณสุขศาสตร์ มหาวิทยาลัยมหิดล; 2551. หน้า 57.