Studies on formation and regeneration of Agaricus bitorquis protoplast
2
Issued Date
2023
Copyright Date
1990
Language
eng
File Type
application/pdf
No. of Pages/File Size
vi, 78 leaves : ill. (some col.)
Access Rights
restricted access
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1990
Suggested Citation
Upatham Pengpara Studies on formation and regeneration of Agaricus bitorquis protoplast. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1990. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90448
Title
Studies on formation and regeneration of Agaricus bitorquis protoplast
Alternative Title(s)
การศึกษาการเกิดโปรโตพลาสและการกลับเป็นเส้นใจของเห็ดกระดุมสายพันธุ์ทนร้อน (Agaricus bitorquis)
Author(s)
Advisor(s)
Abstract
The protoplast fusion technique is one of the several ways of mushroom strain improvement. This research deals with the formation and regeneration of A. bitorquis protoplasts. Some factors affecting the libration of Agaricus bitorquis (KB 629) mycelium were investigated to maximize the protoplast yield. The condition of protoplast formation are important in determining the regenerative potential. The 0.6 M mannitol pH 6.8 (Man 6.8) was the best osmotic stabilizer for the formation of them compared with 0.2 M sodium phosphate buffer pH 6.8 (SPB 6.8) and SPB 6.8 containing 0.2, 0.6, 1.0 M NaCl and KCl. The 10-20 days old, cultured mycelium in Malt extract liquid medium (MELM) on shaker gave the highest yield of protoplasts. The combination of 1.5% w/v NHovozym™ 234 and 0.5% w/v β-Glucuronidase at 2-6 hrs incubations time was the suitable condition for the protoplast formation in comparison to various concentration of single or combination of enzyme such as CELLULASE, HEMICELLULASE, CHITINASE, NOVOZYM TM 234 and β-Glucuronidase Regeneration of protoplast was shown to be influenced by the status of media, only in liquid regeneration medium (LRM) at 35 degree C and in malt extract liquid medium (MELM) later.
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University